Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone
dc.contributor.author | Thaver, Veneesha | |
dc.contributor.author | Meyer, Jacobus Johannes Marion | |
dc.contributor.author | Cockeran, Riana | |
dc.contributor.author | Cholo, Moloko C. | |
dc.contributor.author | Anderson, Ronald | |
dc.contributor.author | Lall, Namrita | |
dc.contributor.email | riana.cockeran@up.ac.za | en_ZA |
dc.date.accessioned | 2016-09-07T07:18:51Z | |
dc.date.available | 2016-09-07T07:18:51Z | |
dc.date.issued | 2012-05 | |
dc.description.abstract | Although the naphthoquinone, 7-methyljuglone (7-MJ), is active against Mycobacterium tuberculosis (MTB) in vitro, neither the cellular site nor mechanism of anti-mycobacterial action of this agent has been identified. The primary objective of the current study was to investigate the mycobacterial outer membrane as a potential target of 7-MJ by measuring the effects of this agent (0.023 - 1.5 mg/L) on microbial ATP levels and uptake of K+. Methods: Bioluminescence and radiometric (uptake of 86Rb+) procedures were used to assay microbial ATP levels and K+ trans-port respectively. Results: Exposure of MTB (strain H37Rv) to 7-MJ for 60 min resulted in dose-related decreases in both microbial ATP levels and uptake of 86Rb+ which achieved statistical significance (P < 0.05) at concentrations of 0.4 and 0.1 mg/L respectively. Conclusions: These observations are compatible with the mycobacterial membrane as being the putative site of action of 7-MJ, targeting microbial energy metabolism and K+ transport. | en_ZA |
dc.description.department | Immunology | en_ZA |
dc.description.department | Plant Science | en_ZA |
dc.description.librarian | am2016 | en_ZA |
dc.description.sponsorship | The National Research Foundation and the Medical Research Council, Pretoria, South Africa. | en_ZA |
dc.description.uri | http://www.SciRP.org/journal/ojrd) | en_ZA |
dc.identifier.citation | Thaver, V, Meyer, JJM, Cockeran, R, Cholo, MC, Anderson, R & Lall, N 2012, 'Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone', Open Journal of Respiratory Diseases, vol. 2, no. 2, pp. 60-62. | en_ZA |
dc.identifier.issn | 2163-940X (print) | |
dc.identifier.issn | 2163-9418 (online) | |
dc.identifier.other | 10.4236/ojrd.2012.22009 | |
dc.identifier.uri | http://hdl.handle.net/2263/56642 | |
dc.language.iso | en | en_ZA |
dc.publisher | Scientific Research Publishing | en_ZA |
dc.rights | © 2012 SciRes | en_ZA |
dc.subject | Adenosine triphosphate | en_ZA |
dc.subject | Cell membrane | en_ZA |
dc.subject | Energy metabolism | en_ZA |
dc.subject | Potassium | en_ZA |
dc.subject | Mycobacterium tuberculosis (MTB) | en_ZA |
dc.title | Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone | en_ZA |
dc.type | Article | en_ZA |