Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone

dc.contributor.authorThaver, Veneesha
dc.contributor.authorMeyer, Jacobus Johannes Marion
dc.contributor.authorCockeran, Riana
dc.contributor.authorCholo, Moloko C.
dc.contributor.authorAnderson, Ronald
dc.contributor.authorLall, Namrita
dc.contributor.emailriana.cockeran@up.ac.zaen_ZA
dc.date.accessioned2016-09-07T07:18:51Z
dc.date.available2016-09-07T07:18:51Z
dc.date.issued2012-05
dc.description.abstractAlthough the naphthoquinone, 7-methyljuglone (7-MJ), is active against Mycobacterium tuberculosis (MTB) in vitro, neither the cellular site nor mechanism of anti-mycobacterial action of this agent has been identified. The primary objective of the current study was to investigate the mycobacterial outer membrane as a potential target of 7-MJ by measuring the effects of this agent (0.023 - 1.5 mg/L) on microbial ATP levels and uptake of K+. Methods: Bioluminescence and radiometric (uptake of 86Rb+) procedures were used to assay microbial ATP levels and K+ trans-port respectively. Results: Exposure of MTB (strain H37Rv) to 7-MJ for 60 min resulted in dose-related decreases in both microbial ATP levels and uptake of 86Rb+ which achieved statistical significance (P < 0.05) at concentrations of 0.4 and 0.1 mg/L respectively. Conclusions: These observations are compatible with the mycobacterial membrane as being the putative site of action of 7-MJ, targeting microbial energy metabolism and K+ transport.en_ZA
dc.description.departmentImmunologyen_ZA
dc.description.departmentPlant Scienceen_ZA
dc.description.librarianam2016en_ZA
dc.description.sponsorshipThe National Research Foundation and the Medical Research Council, Pretoria, South Africa.en_ZA
dc.description.urihttp://www.SciRP.org/journal/ojrd)en_ZA
dc.identifier.citationThaver, V, Meyer, JJM, Cockeran, R, Cholo, MC, Anderson, R & Lall, N 2012, 'Investigation of the anti-mycobacterial mechanism of action of 7-methyljuglone', Open Journal of Respiratory Diseases, vol. 2, no. 2, pp. 60-62.en_ZA
dc.identifier.issn2163-940X (print)
dc.identifier.issn2163-9418 (online)
dc.identifier.other10.4236/ojrd.2012.22009
dc.identifier.urihttp://hdl.handle.net/2263/56642
dc.language.isoenen_ZA
dc.publisherScientific Research Publishingen_ZA
dc.rights© 2012 SciResen_ZA
dc.subjectAdenosine triphosphateen_ZA
dc.subjectCell membraneen_ZA
dc.subjectEnergy metabolismen_ZA
dc.subjectPotassiumen_ZA
dc.subjectMycobacterium tuberculosis (MTB)en_ZA
dc.titleInvestigation of the anti-mycobacterial mechanism of action of 7-methyljugloneen_ZA
dc.typeArticleen_ZA

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