The effects of clofazimine on mycobacterium smegmatis biofilm formation

dc.contributor.advisorCholo, Moloko C.
dc.contributor.emailt.mothiba@yahoo.comen
dc.contributor.postgraduateMothiba, Maborwa Tebogo
dc.date.accessioned2013-09-10T07:01:00Z
dc.date.available2013en
dc.date.available2013-09-10T07:01:00Z
dc.date.created2013en
dc.date.issued2013en
dc.date.submitted2013-07-05en
dc.descriptionDissertation (MSc)--University of Pretoria, 2013.en
dc.description.abstractChemotherapy of tuberculosis (TB), a disease caused by Mycobacterium tuberculosis (M. tuberculosis), is successful against actively-growing bacilli but ineffective against dormant/persistent organisms, found mainly in a protective lipid-laden granuloma, possibly necessitating the use of lipophilic antibiotics. In vitro, these bacilli are encased in lipid-rich biofilms. In this study, the antimycobacterial activity of one such agent, clofazimine, and its nanoparticle formulation, have been investigated against Mycobacterium smegmatis (M. smegmatis), as a surrogate for M. tuberculosis, by determining the bacteriostatic and bactericidal activities of the native (NC) and spray-dried (SDC) preparations of this agent on planktonic and biofilm populations, as well as their effects on biofilm formation and its lipid compositions, specifically free mycolic acid (FM) content. Both preparations were comparable, being bacteriostatic for rapidly-proliferating bacilli, bactericidal for slow-growing, biofilm-producing sessile bacteria, but ineffective against non-replicating, biofilm-encased M. smegmatis organisms. However, similar studies in M. tuberculosis are required.en
dc.description.availabilityUnrestricteden
dc.description.departmentImmunologyen
dc.identifier.citationMothiba, MT 2013, The effects of clofazimine on mycobacterium smegmatis biofilm formation, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd<http://hdl.handle.net/2263/31569 >en
dc.identifier.otherC13/9/948/gm
dc.identifier.urihttp://hdl.handle.net/2263/31569
dc.language.isoenen
dc.publisherUniversity of Pretoria
dc.rights© 2000, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.en
dc.subjectFree mycolic aciden
dc.subjectBiofilmen
dc.subjectPlanktonicen
dc.subjectAntimycobacterial activityen
dc.subjectNanoparticleen
dc.subjectClofazimineen
dc.subjectChemotherapyen
dc.subjectGranulomaen
dc.subjectMycobacterium smegmatisen
dc.subjectMycobacterium tuberculosisen
dc.titleThe effects of clofazimine on mycobacterium smegmatis biofilm formationen
dc.typeDissertationen

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