Molecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive children

dc.contributor.authorBlignaut, Elaine
dc.contributor.authorVan Heerden, Willem Francois Petrus
dc.contributor.emailelaine.blignaut@up.ac.zaen_ZA
dc.date.accessioned2015-08-03T12:34:13Z
dc.date.available2015-08-03T12:34:13Z
dc.date.issued2015-10
dc.description.abstractCandida albicans and caries are frequently investigated among healthy and immunosuppressed individuals. The objective of this study was to demonstrate the presence of C. albicans on both oral soft and hard tissue and to investigate, at molecular level, the genetic subtype of the organism from the two oral sites. Tongue swabs and dentine scrapings from 362 HIV-positive children, referred for the extraction of carious primary teeth, were cultured on CHROMagar and identified to species level with ID32C. Histological staining of extracted carious teeth was also done. In patients with positive C. albicans cultures from both the tongue and carious dentine, DNA fingerprinting of such paired isolates was performed, using Southern blot hybridisation with the Ca3 probe. Yeasts were cultured from the tongue of 151 (41.7 %) individuals and 57 (37.7 %) simultaneously yielded positive C. albicans cultures from carious dentine. Nine different yeast spp. were identified from the tongue using the ID32C commercial system, but C. albicans was the only species recovered from carious dentine and histological investigation demonstrated fungal elements penetrated into the dentine and not limited to superficial debris on the floor of the cavity. Twelve of 13 paired isolates of C. albicans revealed identical fingerprinting patterns. The findings from this study demonstrated that in a particular individual, the same genetic subtype of C. albicans was capable of colonising both oral soft tissue and carious dentine. This renders carious teeth a constant source, or reservoir, of potentially infectious agents and, particularly among immunosuppressed individuals, should therefore not be left unattended.en_ZA
dc.description.embargo2016-10-13en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipMRC granten_ZA
dc.description.urihttp://link.springer.com/journal/11046en_ZA
dc.identifier.citationBlignaut, E & Van Heerden, WFP 2015, 'Molecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive children', Mycopathologia, vol. 180, no. 3, pp. 193-201.en_ZA
dc.identifier.issn0301-486X (print)
dc.identifier.issn1573-0832 (online)
dc.identifier.other10.1007/s11046-015-9912-3
dc.identifier.urihttp://hdl.handle.net/2263/49247
dc.language.isoenen_ZA
dc.publisherSpringeren_ZA
dc.rights© Springer Science+Business Media Dordrecht 2015. The original publication is available at : http://link.springer.comjournal/11046.en_ZA
dc.subjectCandida albicansen_ZA
dc.subjectDNA fingerprintingen_ZA
dc.subjectDeoxyribonucleic acid (DNA)en_ZA
dc.subjectCarious teethen_ZA
dc.subjectHuman immunodeficiency virus (HIV)en_ZA
dc.subjectPaediatric HIV patientsen_ZA
dc.subjectChildrenen_ZA
dc.subjectCariesen_ZA
dc.subjectFungal colonisationen_ZA
dc.subjectCarious dentineen_ZA
dc.titleMolecular and histological association between Candida albicans from oral soft tissue and carious dentine of HIV-positive childrenen_ZA
dc.typePostprint Articleen_ZA

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