Acetone leaf extracts of some South African trees with high activity against Escherichia trees with high activity against Escherichial activity and selectivity index

dc.contributor.authorElisha, Ishaku Leo
dc.contributor.authorBotha, Francien Susanna
dc.contributor.authorMadikizela, Balungile
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorEloff, Jacobus Nicolaas
dc.date.accessioned2017-07-26T13:03:51Z
dc.date.available2017-07-26T13:03:51Z
dc.date.issued2017-06-19
dc.descriptionThis manuscript is part of the output of a Ph.D. research project.en_ZA
dc.description.abstractBACKGROUND : Tuberculosis is a world-wide problem affecting humans and animals. There is increasing development of resistance of the pathogens to current antimycobacterial agents. Many authors have investigated activities of extracts and isolated compounds from plants. The traditional uses of plants have frequently been the criterion to select plants investigated. In this contribution, we investigate whether plant extracts with very good activity against Escherichia coli may also be active against mycobacteria. METHODS : The antimycobacterial activity of eight South African tree leaf extracts with high activity against Escherichia coli were determined in vitro against Mycobacterium smegmatis, M. fortuitum and M. aurum, using a serial microdilution method. The cellular cytotoxicity was also determined by the MTT assay using Vero monkey kidney cells. The selectivity index was determined by dividing the cytotoxicity of extracts by MIC. RESULTS : The antimycobacterial activity of the extracts ranged from 0.02 to 2.5mg/ml. Mycobacterium smegmatis was more sensitive to the extracts (Average MIC=0.96mg/ml) and Mycobacterium aurum was comparatively resistant (Average MIC=2.04mg/ml). The extracts of Cremaspora triflora had strong antimycobacterial activity with a MIC of 0.05mg/ml that compared reasonably well with that of streptomycin (0.01mg/ml) and rifampicin (0.03mg/ml), p>0.05. Cremaspora triflora had the best selectivity index of 2.87 against Mycobacterium fortuitum. CONCLUSION : The high activity of C. triflora extracts against the fast-growing mycobacteria and good cellular safety is promising. It may be interesting to investigate extracts against pathogenic M. tuberculosis, M. bovis and M. avium cultures and to isolate active antimycobacterial compounds.en_ZA
dc.description.departmentParaclinical Sciencesen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe National Research Foundation of South Africa (Eloff IPPR 953991) the Medical Research Council of South Africa (SIR McGaw) and the University of Pretoria.en_ZA
dc.description.urihttp://www.biomedcentral.com/bmccom/plementalternmeden_ZA
dc.identifier.citationElisha, IL, Botha, FS, Madikizela, B, McGaw, LJ & Eloff, JN 2017, 'Acetone leaf extracts of some South African trees with high activity against Escherichia trees with high activity against Escherichial activity and selectivity index', BMC Complementary and Alternative Medicine, vol. 17, art. no. 327, pp. 1-5.en_ZA
dc.identifier.issn1472-6882
dc.identifier.other10.1186/s12906-017-1831-z
dc.identifier.urihttp://hdl.handle.net/2263/61450
dc.language.isoenen_ZA
dc.publisherBioMed Centralen_ZA
dc.rights© The Author(s). 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectAntimycobacterialen_ZA
dc.subjectPlant extractsen_ZA
dc.subjectMinimum inhibitory concentrationen_ZA
dc.subjectSelectivity indexen_ZA
dc.titleAcetone leaf extracts of some South African trees with high activity against Escherichia trees with high activity against Escherichial activity and selectivity indexen_ZA
dc.typeArticleen_ZA

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