Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species

dc.contributor.authorSuleiman, M.M. (Mohammed Musa)
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorNaidoo, Vinny
dc.contributor.authorEloff, Jacobus Nicolaas
dc.contributor.emailkobus.eloff@up.ac.zaen
dc.date.accessioned2010-12-10T07:49:00Z
dc.date.available2010-12-10T07:49:00Z
dc.date.issued2010
dc.description.abstractThe hexane, acetone, dichloromethane and methanol extracts of Combretum vendae A.E. van Wyk (Combretaceae), Commiphora harveyi (Engl.) Engl. (Burseraceae), Khaya anthotheca (Welm.) C.DC (Meliaceae), Kirkia wilmsii Engl. (Kirkiaceae), Loxostylis alata A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia (Bernh. Ex C. Krauss) Engl. (Anacardiaceae) were screened for their antimicrobial activity. The test organisms included bacteria (Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus), and fungi (Aspergillus fumigatus, Candida albicans, Cryptococcus neoformans, Microsporum canis and Sporothrix schenckii). A simple bioautographic procedure, involving spraying suspensions of the bacteria or fungi on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial and antifungal compounds present in the extracts. All the extracts had antimicrobial activity against at least one of the test microorganisms. This activity was denoted by white spots against a red-purple background on the TLC plates after spraying with tetrazolium violet. Twenty seven TLC plates; 9 for each solvent system and 3 different solvent systems per organism were tested in the bioautographic procedure. Of the bacteria tested, S. aureus was inhibited by the most compounds separated on the TLC plates from all the tested plants. Similarly, growth of the fungus C. neoformans was also inhibited by many compounds present in the extracts. Loxostylis alata appeared to be the plant extract with the highest number of inhibition bands when compared with other plants tested against both bacteria and fungi. This species was selected for in depth further study.en
dc.identifier.citationSuleiman, MM, McGaw, LJ, Naidoo, V & Eloff, JN 2010, 'Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species', African Journal of Traditional Complementary and Alternative Medicines, vol. 7, no. 1, pp. 64-78. http://journals.sfu.ca/africanem/index.php/ajtcam]en
dc.identifier.issn0189-6016
dc.identifier.other55952645400
dc.identifier.other7005589445
dc.identifier.other8621439700
dc.identifier.otherA-1508-2008
dc.identifier.urihttp://hdl.handle.net/2263/15417
dc.language.isoenen
dc.publisherAfrican Networks on Ethnomedicinesen
dc.rightsAfrican Networks on Ethnomedicinesen
dc.subjectBioautographyen
dc.subjectAntifungalen
dc.subjectAntibacterialen
dc.subjectSynergismen
dc.subject.lcshAntibacterial agents -- South Africaen
dc.subject.lcshMedicinal plants -- South Africaen
dc.subject.lcshAnti-infective agentsen
dc.titleDetection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree speciesen
dc.typeArticleen

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