Microdiverse bacterial clades prevail across Antarctic wetlands

dc.contributor.authorQuiroga, María V.
dc.contributor.authorStegen, James C.
dc.contributor.authorMataloni, Gabriela
dc.contributor.authorCowan, Don A.
dc.contributor.authorLebre, Pedro Humberto
dc.contributor.authorValverde, Angel
dc.date.accessioned2024-01-22T10:38:17Z
dc.date.available2024-01-22T10:38:17Z
dc.date.issued2024-01
dc.descriptionDATA AVAILABILITY STATEMENT : The sequence data are publicly available at NCBI BioProject database (ID PRJNA719989, 64 sequence data links, https://www.ncbi.nlm.nih.gov/bioproject/PRJNA719989). R code for nearest taxon distance (NTD) and nucleotide similarity of β-nearest ASV indexes, and the modified version of feature-level βNTI index are available at GitHub (https://github.com/mvquiroga/NullModels).en_US
dc.description.abstractAntarctica's extreme environmental conditions impose selection pressures on microbial communities. Indeed, a previous study revealed that bacterial assemblages at the Cierva Point Wetland Complex (CPWC) are shaped by strong homogeneous selection. Yet which bacterial phylogenetic clades are shaped by selection processes and their ecological strategies to thrive in such extreme conditions remain unknown. Here, we applied the phyloscore and feature-level βNTI indexes coupled with phylofactorization to successfully detect bacterial monophyletic clades subjected to homogeneous (HoS) and heterogenous (HeS) selection. Remarkably, only the HoS clades showed high relative abundance across all samples and signs of putative microdiversity. The majority of the amplicon sequence variants (ASVs) within each HoS clade clustered into a unique 97% sequence similarity operational taxonomic unit (OTU) and inhabited a specific environment (lotic, lentic or terrestrial). Our findings suggest the existence of microdiversification leading to sub-taxa niche differentiation, with putative distinct ecotypes (consisting of groups of ASVs) adapted to a specific environment. We hypothesize that HoS clades thriving in the CPWC have phylogenetically conserved traits that accelerate their rate of evolution, enabling them to adapt to strong spatio-temporally variable selection pressures. Variable selection appears to operate within clades to cause very rapid microdiversification without losing key traits that lead to high abundance. Variable and homogeneous selection, therefore, operate simultaneously but on different aspects of organismal ecology. The result is an overall signal of homogeneous selection due to rapid within-clade microdiversification caused by variable selection. It is unknown whether other systems experience this dynamic, and we encourage future work evaluating the transferability of our results.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-15:Life on landen_US
dc.description.sponsorshipANPCyT - Argentina; European Union; Instituto Antártico Argentino - Dirección Nacional del Antártico; Junta de Castilla y León; NRF - South Africa; U.S. Department of Energy-BER program.en_US
dc.description.urihttps://wileyonlinelibrary.com/journal/mecen_US
dc.identifier.citationQuiroga, M.V., Stegen, J.C., Mataloni, G., Cowan, D., Lebre, P.H., & Valverde, A. (2024). Microdiversebacterial clades prevail across Antarctic wetlands. Molecular Ecology, 33, e17189. https://doi.org/10.1111/mec.17189.en_US
dc.identifier.issn0962-1083 (print)
dc.identifier.issn1365-294X (online)
dc.identifier.other10.1111/mec.17189.
dc.identifier.urihttp://hdl.handle.net/2263/94047
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2023 The Authors. Molecular Ecology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License.en_US
dc.subjectAntarcticaen_US
dc.subjectHomogeneous selectionen_US
dc.subjectMicrodiversityen_US
dc.subjectNull modelsen_US
dc.subjectAmplicon sequence variants (ASVs)en_US
dc.subjectHomogeneous (HoS)en_US
dc.subjectHeterogenous (HeS)en_US
dc.subjectOperational taxonomic unit (OTU)en_US
dc.subjectCierva Point Wetland Complex (CPWC)en_US
dc.subjectSDG-15: Life on landen_US
dc.titleMicrodiverse bacterial clades prevail across Antarctic wetlandsen_US
dc.typeArticleen_US

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