The development of Cowdria ruminantium in neutrophils

dc.contributor.authorLogan, L.L.
dc.contributor.authorWhyard, T.C.
dc.contributor.authorQuintero, J.C.
dc.contributor.authorMebus, C.A.
dc.contributor.editorBigalke, R.D.
dc.date.accessioned2017-11-13T09:23:41Z
dc.date.available2017-11-13T09:23:41Z
dc.date.created2017
dc.date.issued1987
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en_ZA
dc.description.abstractThe sequential development of C. ruminantium (Kwanyanga and Kumm isolates) was followed in caprine leukocyte cultures by light microscopy, direct immunofluorescent microscopy (DFA), indirect immunofluorescent microscopy (IFA) and transmission electron microscopy (TEM). During the febrile response, one to several small cocci, large ring forms or rods were observed in neutrophils in blood smears and cytopreparations of neutrophil fractions using Diff Quik stain, Giemsa stain, DFA and TEM. One to several C. ruminantium colonies were seen in up to 35% of neutrophils maintained in vitro for 18 h to 5 days. The organisms were located in neutrophil phagosomes by TEM and were enveloped by two trilamellar unit membranes. Initially, C. ruminantium was tightly enclosed within phagosomes. At 20 h of incubation, organisms were frequently observed undergoing binary fission within enlarged phagosomal vacuoles. At later time periods, neutrophils harboured fully formed colonies (morula) containing numerous organisms. An occasional C. ruminantium-infected macrophage (Kumm isolate), and an occasional infected eosinophil (Kumm and Kwanyanga isolate) were found.en_ZA
dc.identifier.citationLogan, LL, Whyard, TC, Quintero, JC & Mebus, CA 1987, 'The development of Cowdria ruminantium in neutrophils', Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 197-204.en_ZA
dc.identifier.issn0330-2465
dc.identifier.urihttp://hdl.handle.net/2263/63119
dc.language.isoenen_ZA
dc.publisherPublished by the Government Printer, Pretoria.en_ZA
dc.rights©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital).en_ZA
dc.subjectVeterinary medicineen_ZA
dc.subject.lcshVeterinary medicine -- South Africa
dc.titleThe development of Cowdria ruminantium in neutrophilsen_ZA
dc.title.alternativeHeartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986en_ZA
dc.typeArticleen_ZA

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