Benzimidazole-resistant Haemonchus contortus-the effect of cryopreservation on the resistance of Successive generations
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Date
Authors
Van Wyk, Jan Aucamp
Gerber, H.M.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Government Printer, Pretoria.
Abstract
Infective larvae (L3) of a strain of benzimidazole-resistant Haemonchus contortus were stored in the gas phase of liquid nitrogen, thawed and used to infest worm-free sheep from which, in turn, larvae were cultured, frozen and thawed. This cycle was repeated 5 times. Thereafter, the progeny of the cryopreserved larvae were compared with the progeny of the original untreated larvae for susceptibility to benzimidazole anthelmintics.
Repeated freezing of consecutive generations of L3 in liquid nitrogen did not appear to affect their relative resistance to benzimidazoles and, although other strains must also be tested, it would seem that cryopreservation may be used for storing resistant strains.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Van Wyk, JA & Gerber, HM 1980, 'Benzimidazole-resistant Haemonchus contortus - the effect of cryopreservation on the resistance of successive generations.’, Onderstepoort Journal of Veterinary Research, vol. 47, no. 3, pp. 143-146.