Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus

dc.contributor.authorMizrachi, Eshchar
dc.contributor.authorMaloney, Victoria J.
dc.contributor.authorSilberbauer, Janine Francina
dc.contributor.authorHefer, Charles Amadeus
dc.contributor.authorBerger, David Kenneth
dc.contributor.authorMansfield, Shawn D.
dc.contributor.authorMyburg, Alexander Andrew
dc.date.accessioned2015-12-11T07:49:03Z
dc.date.issued2015-06
dc.description.abstractTension wood has distinct physical and chemical properties, including altered fibre properties, cell wall composition and ultrastructure. It serves as a good system for investigating the genetic regulation of secondary cell wall biosynthesis and wood formation. The reference genome sequence for Eucalyptus grandis allows investigation of the global transcriptional reprogramming that accompanies tension wood formation in this global wood fibre crop. We report the first comprehensive analysis of physicochemical wood property changes in tension wood of Eucalyptus measured in a hybrid (E. grandis 9 Eucalyptus urophylla) clone, as well as genome-wide gene expression changes in xylem tissues 3wk post-induction using RNA sequencing. We found that Eucalyptus tension wood in field-grown trees is characterized by an increase in cellulose, a reduction in lignin, xylose and mannose, and a marked increase in galactose. Gene expression profiling in tension wood-forming tissue showed corresponding down-regulation of monolignol biosynthetic genes, and differential expression of several carbohydrate active enzymes. We conclude that alterations of cell wall traits induced by tension wood formation in Eucalyptus are a consequence of a combination of down-regulation of lignin biosynthesis and hemicellulose remodelling, rather than the often proposed up-regulation of the cellulose biosynthetic pathway.en_ZA
dc.description.embargo2016-06-30
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipSouth African Department of Science and Technology (DST), Sappi and Mondi, through the Forest Molecular Genetics Programme, the Technology and Human Resources for Industry Programme (THRIP, UID 80118) and the Bioinformatics and Functional Genomics Programme of the National Research Foundation (NRF, UID 18312) of South Africa.en_ZA
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137en_ZA
dc.identifier.citationMizrachi, E, Maloney, VJ, Silberbauer, J, Hefer, CA, Berger, DK, Mansfield, SD & Myburg, AA 2015, 'Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus', New Phytologist, vol. 206, no. 4, pp. 1351-1363.en_ZA
dc.identifier.issn0028-646X (print)
dc.identifier.issn1469-8137 (online)
dc.identifier.other10.1111/nph.13152
dc.identifier.urihttp://hdl.handle.net/2263/51151
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2014 New Phytologist Trust. This is the pre-peer reviewed version of the following article : Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus, New Phytologist, vol. 206, no. 4, pp. 1351-1363 SI, 2015. doi : 10.1111/nph.13152. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1469-8137.en_ZA
dc.subjectCelluloseen_ZA
dc.subjectEucalyptusen_ZA
dc.subjectHhemicelluloseen_ZA
dc.subjectLigninen_ZA
dc.subjectRNA-seqen_ZA
dc.subjectTension wooden_ZA
dc.subjectTranscriptomeen_ZA
dc.subjectXylanen_ZA
dc.titleInvestigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptusen_ZA
dc.typePostprint Articleen_ZA

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