Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus
dc.contributor.author | Mizrachi, Eshchar | |
dc.contributor.author | Maloney, Victoria J. | |
dc.contributor.author | Silberbauer, Janine Francina | |
dc.contributor.author | Hefer, Charles Amadeus | |
dc.contributor.author | Berger, David Kenneth | |
dc.contributor.author | Mansfield, Shawn D. | |
dc.contributor.author | Myburg, Alexander Andrew | |
dc.date.accessioned | 2015-12-11T07:49:03Z | |
dc.date.issued | 2015-06 | |
dc.description.abstract | Tension wood has distinct physical and chemical properties, including altered fibre properties, cell wall composition and ultrastructure. It serves as a good system for investigating the genetic regulation of secondary cell wall biosynthesis and wood formation. The reference genome sequence for Eucalyptus grandis allows investigation of the global transcriptional reprogramming that accompanies tension wood formation in this global wood fibre crop. We report the first comprehensive analysis of physicochemical wood property changes in tension wood of Eucalyptus measured in a hybrid (E. grandis 9 Eucalyptus urophylla) clone, as well as genome-wide gene expression changes in xylem tissues 3wk post-induction using RNA sequencing. We found that Eucalyptus tension wood in field-grown trees is characterized by an increase in cellulose, a reduction in lignin, xylose and mannose, and a marked increase in galactose. Gene expression profiling in tension wood-forming tissue showed corresponding down-regulation of monolignol biosynthetic genes, and differential expression of several carbohydrate active enzymes. We conclude that alterations of cell wall traits induced by tension wood formation in Eucalyptus are a consequence of a combination of down-regulation of lignin biosynthesis and hemicellulose remodelling, rather than the often proposed up-regulation of the cellulose biosynthetic pathway. | en_ZA |
dc.description.embargo | 2016-06-30 | |
dc.description.librarian | hb2015 | en_ZA |
dc.description.sponsorship | South African Department of Science and Technology (DST), Sappi and Mondi, through the Forest Molecular Genetics Programme, the Technology and Human Resources for Industry Programme (THRIP, UID 80118) and the Bioinformatics and Functional Genomics Programme of the National Research Foundation (NRF, UID 18312) of South Africa. | en_ZA |
dc.description.uri | http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1469-8137 | en_ZA |
dc.identifier.citation | Mizrachi, E, Maloney, VJ, Silberbauer, J, Hefer, CA, Berger, DK, Mansfield, SD & Myburg, AA 2015, 'Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus', New Phytologist, vol. 206, no. 4, pp. 1351-1363. | en_ZA |
dc.identifier.issn | 0028-646X (print) | |
dc.identifier.issn | 1469-8137 (online) | |
dc.identifier.other | 10.1111/nph.13152 | |
dc.identifier.uri | http://hdl.handle.net/2263/51151 | |
dc.language.iso | en | en_ZA |
dc.publisher | Wiley | en_ZA |
dc.rights | © 2014 New Phytologist Trust. This is the pre-peer reviewed version of the following article : Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus, New Phytologist, vol. 206, no. 4, pp. 1351-1363 SI, 2015. doi : 10.1111/nph.13152. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1469-8137. | en_ZA |
dc.subject | Cellulose | en_ZA |
dc.subject | Eucalyptus | en_ZA |
dc.subject | Hhemicellulose | en_ZA |
dc.subject | Lignin | en_ZA |
dc.subject | RNA-seq | en_ZA |
dc.subject | Tension wood | en_ZA |
dc.subject | Transcriptome | en_ZA |
dc.subject | Xylan | en_ZA |
dc.title | Investigating the molecular underpinnings underlying morphology and changes in carbon partitioning during tension wood formation in Eucalyptus | en_ZA |
dc.type | Postprint Article | en_ZA |