Genomic characterization of two novel viruses infecting Barleria cristata L. from the genera Orthotospovirus and Polerovirus

Read, David Alan; Roberts, Ronel; Thompson, Genevieve Dawn

Genomic characterization of two novel viruses infecting Barleria cristata L. from the genera Orthotospovirus and Polerovirus

dc.contributor.author	Read, David Alan
dc.contributor.author	Roberts, Ronel
dc.contributor.author	Thompson, Genevieve Dawn
dc.contributor.email	david.read@fabi.up.ac.za	en_ZA
dc.date.accessioned	2022-03-04T05:03:52Z
dc.date.issued	2021-09
dc.description	SUPPLEMENTARY INFORMATION : Supplementary Figure 1: Foliar symptoms associated with Barleria cristata plants that were sampled in this study. Large, diffuse chlorotic spots were associated with the single infection of barleria severe mosaic virus (BSMoV) (19-3031), while a more defined mosaic was associated with the mixed involving both BSMoV and barleria polerovirus 1 (19-3037).	en_ZA
dc.description	Supplementary Figure 2: Maximum likelihood phylogeny based on the amino acid sequences of the N-protein of barleria chlorosis-associated virus (indicated by solid circle markers) and selected members of the Tospoviridae family. The phylogeny represents the tree with the highest log likelihood and was generated in MEGA X using the best-fit (Le Gascuel) model with gamma distribution (n=4). Bootstrapping was applied (1000 replicates) and the percentage of trees in which the associated taxa clustered together is shown next to the branches. Bootstrap percentages lower than 50 are not shown. The cognate amino acid sequence of Guaroa virus was used as an outgroup.	en_ZA
dc.description	Supplementary Figure 3: Maximum likelihood phylogeny based on the amino acid sequences of the RNA-dependant RNA polymerase of barleria polero virus 1 (indicated by solid circle markers) and selected members of the Luteoviridae family. The phylogeny represents the tree with the highest log likelihood and was generated in MEGA X using the best-fit (Jones-Taylor-Thornton) model. Bootstrapping was applied (1000 replicates) and the percentage of trees in which the associated taxa clustered together is shown next to the branches. Bootstrap percentages lower than 50 are not shown. The cognate amino acid sequences of two enamoviruses were used as outgroups.	en_ZA
dc.description.abstract	Barleria cristata L. has become naturalized in South Africa, where it is commonly used as an ornamental. In 2019, plants of B. cristata showing putative viral symptoms were collected from two locations in Gauteng, South Africa. RNAtag-seq libraries were prepared and sequenced using an Illumina HiSeq 2500 platform. De novo assembly of the resulting data revealed the presence of a novel member of the family Tospoviridae associated with the plants from both locations, and this virus was given the tentative name "barleria chlorosis-associated virus". Segments L, M, and S have lengths of 8752, 4760, and 2906 nt, respectively. Additionally, one of the samples was associated with a novel polerovirus, provisionally named "barleria polerovirus 1", with a complete genome length of 6096 nt. This is the first study to show the association of viruses with a member of the genus Barleria.	en_ZA
dc.description.department	Forestry and Agricultural Biotechnology Institute (FABI)	en_ZA
dc.description.embargo	2022-07-01
dc.description.librarian	hj2022	en_ZA
dc.description.uri	http://link.springer.com/journal/705	en_ZA
dc.identifier.citation	Read, D.A., Roberts, R. & Thompson, G. Genomic characterization of two novel viruses infecting Barleria cristata L. from the genera Orthotospovirus and Polerovirus. Archives of Virology 166, 2615–2618 (2021). https://doi.org/10.1007/s00705-021-05150-7.	en_ZA
dc.identifier.issn	0304-8608 (print)
dc.identifier.issn	1432-8798 (online)
dc.identifier.other	10.1007/s00705-021-05150-7
dc.identifier.uri	http://hdl.handle.net/2263/84332
dc.language.iso	en	en_ZA
dc.publisher	Springer	en_ZA
dc.rights	© 2021, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. The original publication is available at : http://link.springer.comjournal/705.	en_ZA
dc.subject	Barleria cristata L.	en_ZA
dc.subject	South Africa (SA)	en_ZA
dc.subject	Polerovirus	en_ZA
dc.subject	Orthotospovirus	en_ZA
dc.title	Genomic characterization of two novel viruses infecting Barleria cristata L. from the genera Orthotospovirus and Polerovirus	en_ZA
dc.type	Postprint Article	en_ZA