Fungal diversity associated with the mycorrhizosphere soil of Brachycorythis conica subsp. transvaalensis, a critically endangered and endemic terrestrial orchid from South Africa

dc.contributor.authorMakwela, Modjadji Carol
dc.contributor.authorHammerbacher, Almuth
dc.contributor.authorCoetzee, Martin Petrus Albertus
dc.contributor.authorWingfield, Brenda D.
dc.contributor.authorVan Ede, Gerrit
dc.contributor.authorBose, Tanay
dc.contributor.emailalmuth.hammerbacher@fabi.up.ac.zaen_US
dc.date.accessioned2023-07-11T11:47:30Z
dc.date.available2023-07-11T11:47:30Z
dc.date.issued2022-05
dc.descriptionDATA ACCESSIBILITY : The high-throughput sequencing data generated in this study is available at the NCBI Sequence Read Archive (https://submit.ncbi.nlm.nih.gov/ subs/sra/) under the accession number PRJNA693177.en_US
dc.description.abstractThe Albertina Sisulu orchid, Brachycorythis conica subsp. transvaalensis is a critically endangered terrestrial orchid with a single population remaining in the Gauteng Province of South Africa. For the conservation of this endemic orchid, several strategies are being implemented such as protection of habitat, identifying pollinators and in vitro propagation. For symbiotic germination, it is essential to identify the mycorrhizal associates of this orchid using non-destructive sampling. In this study, high-throughput sequencing was used to catalogue and compare the diversity of fungi associated with the mycorrhizosphere of this orchid and non-mycorrhizosphere soils collected from the same coordinates. Bioinformatics and statistical analyses of the data showed that, despite the substantial overlap in the community composition of fungi associated with these two soil types, several exclusive fungal species were identified from the mycorrhizosphere of the orchid. These included an assortment of potential orchid mycorrhizal species from the orders Agaricales, Cantharellales and Sebacinales. This study provides the first insight into the soil fungal diversity associated with the mycorrhizosphere of this critically endangered orchid. In the future, data from this study can be used for optimising conservation measures and isolation of suitable mycorrhizal species required for in vitro symbiotic germination of this orchid.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.departmentZoology and Entomologyen_US
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe NRF Foundational Biodiversity Information Programme, the International Partnergroup Program of the Max Planck Society and the NRF SARChI Chair in Fungal Genomics.en_US
dc.description.urihttp://www.elsevier.com/locate/sajben_US
dc.identifier.citationMakwela, M.C., Hammerbacher, A., Coetzee, M.P. et al. 2022, 'Fungal diversity associated with the mycorrhizosphere soil of Brachycorythis conica subsp. transvaalensis, a critically endangered and endemic terrestrial orchid from South Africa', South African Journal of Botany, vol. 146, pp. 807-814, doi : 10.1016/j.sajb.2022.01.019.en_US
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2022.01.019
dc.identifier.urihttp://hdl.handle.net/2263/91336
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© 2022 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 146, pp. 807-814, 2022, doi : 10.1016/j.sajb.2022.01.019.en_US
dc.subjectAgaricalesen_US
dc.subjectAlbertina Sisulu orchid (Brachycorythis conica)en_US
dc.subjectCantharellalesen_US
dc.subjectOrchid mycorrhizaeen_US
dc.subjectSebacinalesen_US
dc.subjectSDG-15: Life on landen_US
dc.subjectEndangered terrestrial orchiden_US
dc.subjectEndemic terrestrial orchiden_US
dc.subjectSouth Africa (SA)en_US
dc.titleFungal diversity associated with the mycorrhizosphere soil of Brachycorythis conica subsp. transvaalensis, a critically endangered and endemic terrestrial orchid from South Africaen_US
dc.typePostprint Articleen_US

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