A comparative evaluation of the new Genexpert MTB/RIF ultra and other rapid diagnostic assays for detecting tuberculosis in pulmonary and extra pulmonary specimens

dc.contributor.authorOsei Sekyere, John
dc.contributor.authorMaphalala, Nontobeko
dc.contributor.authorMalinga, Lesibana Anthony
dc.contributor.authorMbelle, Nontombi Marylucy
dc.contributor.authorManingi, Nontuthuko Excellent
dc.date.accessioned2020-07-23T09:49:18Z
dc.date.available2020-07-23T09:49:18Z
dc.date.issued2019-11
dc.description.abstractStudies evaluating the new GeneXpert Ultra with other rapid diagnostic assays are limited, particularly in diferent geographical settings. The performance of the GeneXpert Ultra, the GeneXpert G4, the Line probe assays (LPA) and auramine smear microscopy in detecting TB in pulmonary and extrapulmonary samples were thus evaluated. Remnants (n=205 samples) of pulmonary (n=125 samples) and extra-pulmonary (n=80 samples) specimens from TB suspects were prospectively collected. Each sample was divided for diagnosis using microscopy, GeneXpert MTB/RIF assays, and LPA; these were all comparatively evaluated, using the MGIT 960 culture as a gold standard. The sensitivity and specifcity of microscopy, Xpert Ultra, Xpert G4 and MTBDRplus (ver 2) in pulmonary samples were respectively: 82.00% and 90.28%; 88.00% and 58.57%; 79.59% and 90.28%; 80.00% and 11.11%. For extrapulmonary specimen, the sensitivity and specifcity were respectively: 53.85% and 98.51%; 69.23% and 49.25%; 50.00% and 97.01%; 69.23% and 25.37%. The new and improved GeneXpert Ultra assay was more sensitive than GeneXpert G4 and LPA in both pulmonary and extra pulmonary samples, albeit with lower specifcity than the GeneXpert G4. The auramine and LPA tests were also highly sensitive, although the LPA was less specifc.en_ZA
dc.description.departmentMedical Microbiologyen_ZA
dc.description.librarianpm2020en_ZA
dc.description.sponsorshipUniversity of Pretoria; National Research Foundation; Medical Research Council of South Africaen_ZA
dc.description.urihttps://www.nature.com/srepen_ZA
dc.identifier.citationSekyere, J.O., Maphalala, N., Malinga, L.A. et al, 2019, 'A comparative evaluation of the new Genexpert MTB/RIF ultra and other rapid diagnostic assays for detecting tuberculosis in pulmonary and extra pulmonary specimens', Scientific Reports, vol. 9, no.1, art. 16587, pp. 1-9. en_ZA
dc.identifier.issn2045-2322 (online)
dc.identifier.other10.1038/s41598-019-53086-5
dc.identifier.urihttp://hdl.handle.net/2263/75412
dc.language.isoenen_ZA
dc.publisherNature Publising Groupen_ZA
dc.rights© 2019. The Author(s). Licensed under a Creative Commons Attribution 4.0 International License,(http://creativecommons.org/licenses/by/4.0/).en_ZA
dc.subjectBacteriologyen_ZA
dc.subjectClinical microbiologyen_ZA
dc.subjectDiagnostic assaysen_ZA
dc.subjectSpecimenen_ZA
dc.subjectGenexpert MTB/RIF Ultraen_ZA
dc.subjectPulmonaryen_ZA
dc.subjectTuberculosis (TB)en_ZA
dc.titleA comparative evaluation of the new Genexpert MTB/RIF ultra and other rapid diagnostic assays for detecting tuberculosis in pulmonary and extra pulmonary specimensen_ZA
dc.typeArticleen_ZA

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