Isolation and characterization of outer membrane vesicles of pectobacterium brasiliense 1692

dc.contributor.authorMaphosa, Silindile
dc.contributor.authorMoleleki, Lucy N.
dc.contributor.emaillucy.moleleki@up.ac.zaen_US
dc.date.accessioned2022-05-24T10:27:48Z
dc.date.available2022-05-24T10:27:48Z
dc.date.issued2021-09-09
dc.descriptionSUPPLEMENTARY MATERIAL : FIGURE S1: Size distribution and concentration of nanoparticles in cell-free supernatants of exponential and stationary phase Pbr1692 cultures; TABLE S1: Pectobacterium brasiliense 1692 Outer Membrane Vesicle associated proteins.en_US
dc.description.abstractPectobacterium brasiliense (Pbr) 1692 is an aggressive phytopathogen affecting a broad host range of crops and ornamental plants, including potatoes. Previous research on animal pathogens, and a few plant pathogens, revealed that Outer Membrane Vesicles (OMVs) are part of Gram-negative bacteria’s (GNB) adaptive toolkit. For this reason, OMV production and subsequent release from bacteria is a conserved process. Therefore, we hypothesized that OMVs might transport proteins that play a critical role in causing soft rot disease and in the survival and fitness of Pbr1692. Here, we show that the potato pathogen, Pbr1692, releases OMVs of various morphologies in Luria Bertani media at 31 C. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) confirmed the production of OMVs by Pbr1692 cells. Transmission Electron Microscopy showed that these exist as chain-, single-, and double-membrane morphologies. Mass spectrometry followed by Gene Ontology, Clusters of Orthologous Groups, Virulence Factor, CAZymes, Antibiotic Resistance Ontology, and Bastion6 T6SE annotations identified 129 OMV-associated proteins with diverse annotated roles, including antibiotic stress response, virulence, and competition. Pbr1692 OMVs contributed to virulence in potato tubers and elicited a hypersensitive response in Nicotiana benthamiana leaves. Furthermore, Pbr1692 OMVs demonstrated antibacterial activity against Dickeya dadantii.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2022en_US
dc.description.sponsorshipThe National Research Foundation (NRF) Competitive Funding for Rated Researchers (120858) South Africa. and DAAD: German Academic Exchange Service (57408861).en_US
dc.description.urihttps://www.mdpi.com/journal/microorganismsen_US
dc.identifier.citationMaphosa, S.; Moleleki, L.N. Isolation and Characterization of Outer Membrane Vesicles of Pectobacterium brasiliense 1692. Microorganisms 2021, 9, 1918. https://DOI.org/ 10.3390/microorganisms9091918.en_US
dc.identifier.issn2072-666X (online)
dc.identifier.other10.3390/ microorganisms9091918
dc.identifier.urihttps://repository.up.ac.za/handle/2263/85651
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectOuter membrane vesicles (OMVs)en_US
dc.subjectVirulenceen_US
dc.subjectCompetitionen_US
dc.subjectPectobacterium brasiliense (Pbr)en_US
dc.subjectGram-negative bacteria’s (GNB)en_US
dc.subjectScanning electron microscopy (SEM)en_US
dc.subjectTransmission electron microscopy (TEM)en_US
dc.titleIsolation and characterization of outer membrane vesicles of pectobacterium brasiliense 1692en_US
dc.typeArticleen_US

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