Insight into the metabolic profiles of Pb(II) removing microorganisms
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Date
Authors
Cilliers, Carla
Chirwa, Evans M.N.
Brink, Hendrik Gideon
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
The objective of the study was to gather insight into the metabolism of lead-removing
microorganisms, coupled with Pb(II) removal, biomass viability and nitrate concentrations for
Pb(II) bioremoval using an industrially obtained microbial consortium. The consortium used for
study has proven to be highly effective at removing aqueous Pb(II) from solution. Anaerobic
batch experiments were conducted with Luria-Bertani broth as rich growth medium over a period
of 33 h, comparing a lower concentration of Pb(II) with a higher concentration at two different
nutrient concentrations. Metabolite profiling and quantification were conducted with the aid of both
liquid chromatography coupled with tandem mass spectroscopy (UPLC-HDMS) in a “non-targeted”
fashion and high-performance liquid chromatography (HPLC) in a “targeted” fashion. Four main
compounds were identified, and a metabolic study was conducted on each to establish their possible
significance for Pb(II) bioremoval. The study investigates the first metabolic profile to date for Pb(II)
bioremoval, which in turn can result in a clarified understanding for development on an industrial
and microbial level.
Description
Keywords
Pb(II) bio-removal, Metabolic flux analysis, Redox balance, Bioremediation
Sustainable Development Goals
Citation
Cilliers, C.; Chirwa, E.M.N.;
Brink, H.G. Insight into the Metabolic
Profiles of Pb(II) Removing
Microorganisms. Molecules 2021, 26,
4008. https://DOI.org/10.3390/molecules26134008.