Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain

dc.contributor.authorNyanzi, Richard
dc.contributor.authorAwouafack, Maurice D.
dc.contributor.authorSteenkamp, Paul Anton
dc.contributor.authorJooste, Piet J.
dc.contributor.authorEloff, Jacobus Nicolaas
dc.contributor.emailkobus.eloff@up.ac.zaen_ZA
dc.date.accessioned2015-07-14T09:22:32Z
dc.date.available2015-07-14T09:22:32Z
dc.date.issued2014-12
dc.description.abstractThis study investigated the anti-Candida activity of methanol extracts from freeze-dried probiotic cells and the isolation of some constituents in the extracts. The MIC values of the probiotic methanol cell extracts against Candida albicans ranged between 1.25 and 5 mg/ml after 48 h of incubation. However, Lactococcus latics subsp. lactis strain X and Lactobacillus casei strain B extracts had an MIC of 10 mg/ml after 48 h of incubation. The extracts had fungistatic rather than fungicidal activity. These extracts had a much higher antifungal activity than antifungal compounds isolated from the growth medium by many other authors. This indicates that probiotics may also release antifungal compounds in their cells that could contribute to a therapeutic effect. Lactic acid (1) and 6-O-(α-D-glucopyranosyl)-1,6-di-O-pentadecanoyl-α-D-glucopyranose a novel fatty acid derivative (2) were isolated from methanol probiotic extracts and the structure of these compounds were elucidated using NMR (1 and 2D) and mass spectrometry (MS).en_ZA
dc.description.embargo2015-12-31en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipDr. M.D. Awouafack received a Postdoctoral Fellowship from the University of Pretoria to work at the Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science. The Medical Research Council provided funding.en_ZA
dc.description.urihttp://www.elsevier.com/locate/foodchemen_ZA
dc.identifier.citationNyanzi, R, Awouafack, MD, Steenkamp, P, Jooste, P & Eloff, JN 2014, 'Anticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strain', Food Chemistry, vol. 164, pp. 470-475.en_ZA
dc.identifier.issn0308-8146 (print)
dc.identifier.issn1873-7072 (online)
dc.identifier.other10.1016/j.foodchem.2014.05.067
dc.identifier.urihttp://hdl.handle.net/2263/48691
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2014 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Food Chemistry. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Food Chemistry, vol. 164, pp. 470-475, 2014. doi : 10.1016/j.foodchem.2014.05.067en_ZA
dc.subjectProbioticen_ZA
dc.subjectLactobacillusen_ZA
dc.subjectConstituentsen_ZA
dc.subjectAnti-Candidaen_ZA
dc.titleAnticandidal activity of cell extracts from 13 probiotic Lactobacillus strains and characterisation of lactic acid and a novel fatty acid derivative from one strainen_ZA
dc.typePostprint Articleen_ZA

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