TB/HIV pleurisy reduces Th17 lymphocyte proportion independent of the cytokine microenvironment

dc.contributor.authorKorb, Vanessa C.
dc.contributor.authorPhulukdaree, Alisa
dc.contributor.authorLalloo, Umesh G.
dc.contributor.authorChuturgoon, Anil A.
dc.contributor.authorMoodley, Devapregasan
dc.contributor.emailalisa.phulukdaree@up.ac.zaen_ZA
dc.date.accessioned2016-10-12T12:23:00Z
dc.date.issued2016-07
dc.description.abstractT-helper (Th) 17 cells are a pro-inflammatory subset of CD4+ effector T-cells critical in mucosal immunity. Imbalances in Th17 cell proportion have been implicated in the pathogenesis of several diseases; however, this has not been adequately explored in tuberculosis (TB) and human immunodeficiency virus (HIV) co-infection. Since Th17 cells are predominantly mucosally associated, we assessed Th17 proportion and associated microenvironment in pleural effusions from patients co-infected with TB/HIV. Our results show that TB+HIV+ pleurisy results in significantly reduced frequency of CD4+IL-17+RORC+STAT3+ Th17 cells compared to TB−HIV−ex vivo (p = 0.0054) and was confirmed in conditioned media studies in vitro (p = 0.0001). This was not associated with alterations in Th17 polarising cytokines IL-6, IL-21 and IL-23 or changes in Th17 signature cytokines IL-17A and F. However, the mRNA expression of Th17 signalling molecules, IL-6 (p = 0.0022), IL-6R (p = 0.0247), IL-1β (p = 0.0022) and signal transducer and activator (STAT) 3 (p = 0.0022) were significantly upregulated. Notably, TB+HIV+ pleural fluid contained significantly higher concentrations of IL-1β (p = 0.0008), IL-22 (p = 0.0115), IL-31 (p = 0.0210), TNF-α (p = 0.0251) and IFN-γ (p = 0.0026) than TB−HIV− pleural fluid ex vivo. Taken together, this suggests a reduced portion of Th17 lymphocytes in TB/HIV pleurisy is independent of locally mediated cytokine polarisation.en_ZA
dc.description.departmentPhysiologyen_ZA
dc.description.embargo2017-07-31
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipThe National Research Foundation, KwaZulu-Natal Research Institute for Tuberculosis and HIV and College of Health Sciences, University of KwaZulu-Natal.en_ZA
dc.description.urihttp://intl.elsevierhealth.com/journals/tubeen_ZA
dc.identifier.citationKorb, VC, Phulukdaree, A, Lalloo, UGA, Chuturgoon, AA & Moodley, D 2016, 'TB/HIV pleurisy reduces Th17 lymphocyte proportion independent of the cytokine microenvironment', Tuberculosis, vol. 99, pp. 92-99.en_ZA
dc.identifier.issn1472-9792 (print)
dc.identifier.issn1873-281X (online)
dc.identifier.other10.1016/j.tube.2016.05.001
dc.identifier.urihttp://hdl.handle.net/2263/57127
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2016 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Tuberculosis. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Tuberculosis, vol. 99, pp. 92-99, 2016. doi : 10.1016/j.tube.2016.05.001.en_ZA
dc.subjectCo-infectionen_ZA
dc.subjectTh17en_ZA
dc.subjectIL-17en_ZA
dc.subjectTuberculosis (TB)en_ZA
dc.subjectHuman immunodeficiency virus (HIV)en_ZA
dc.subjectIL-1βen_ZA
dc.titleTB/HIV pleurisy reduces Th17 lymphocyte proportion independent of the cytokine microenvironmenten_ZA
dc.typePostprint Articleen_ZA

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