Kisspeptin, neurokinin B, and dynorphin cct in the arcuate nucleus to control activity of the GnRH pulse generator in Ewes

dc.contributor.authorGoodman, Robert L.
dc.contributor.authorHileman, Stanley M.
dc.contributor.authorNestor, Casey C.
dc.contributor.authorPorter, Katrina L.
dc.contributor.authorConnors, John M.
dc.contributor.authorHardy, Steve L.
dc.contributor.authorMillar, Robert P.
dc.contributor.authorCernea, Maria
dc.contributor.authorCoolen, Lique M.
dc.contributor.authorLehman, Michael N.
dc.date.accessioned2014-04-01T09:52:08Z
dc.date.available2014-04-01T09:52:08Z
dc.date.issued2013-11
dc.description.abstractRecent work has led to the hypothesis that kisspeptin/neurokinin B/dynorphin (KNDy) neurons in the arcuate nucleus play a key role in GnRH pulse generation, with kisspeptin driving GnRH release and neurokinin B (NKB) and dynorphin acting as start and stop signals, respectively. In this study, we tested this hypothesis by determining the actions, if any, of four neurotransmitters found in KNDy neurons (kisspeptin, NKB, dynorphin, and glutamate) on episodic LH secretion using local administration of agonists and antagonists to receptors for these transmitters in ovariectomized ewes. We also obtained evidence that GnRH-containing afferents contact KNDy neurons, so we tested the role of two components of these afferents: GnRH and orphanin-FQ. Microimplants of a Kiss1r antagonist briefly inhibited LH pulses and microinjections of 2 nmol of this antagonist produced a modest transitory decrease in LH pulse frequency. An antagonist to the NKB receptor also decreased LH pulse frequency, whereas NKB and an antagonist to the receptor for dynorphin both increased pulse frequency. In contrast, antagonists toGnRHreceptors, orphanin-FQ receptors, and the N-methyl-D-aspartate glutamate receptor had no effect on episodic LH secretion.Wethus conclude that the KNDy neuropeptides act in the arcuate nucleus to control episodic GnRH secretion in the ewe, but afferent input from GnRH neurons to this area does not. These data support the proposed roles forNKBand dynorphin within theKNDyneural network and raise the possibility that kisspeptin contributes to the control ofGnRHpulse frequency in addition to its established role as an output signal from KNDy neurons that drives GnRH pulses.en_US
dc.description.librarianam2014en_US
dc.description.sponsorshipNational Institutes of Health Grants R01-HD039916 and RO1-HD017864.en_US
dc.description.urihttp://press.endocrine.org/journal/endoen_US
dc.identifier.citationGoodman, RL, Hileman, SM, Nestor, CC, Porter, KL, Connors, JM, Hardy, SL, Millar, RP, Cernea, M, Coolen, LM & Lehman, MN 2013, 'Kisspeptin, neurokinin B, and dynorphin cct in the arcuate nucleus to control activity of the GnRH pulse generator in Ewes', Endocrinology, vol. 154, no. 11, pp. 4259-4269.en_US
dc.identifier.issn0013-7227 (print)
dc.identifier.issn1945-7170 (online)
dc.identifier.other10.1210/en.2013-1331
dc.identifier.urihttp://hdl.handle.net/2263/37326
dc.language.isoenen_US
dc.publisherThe Endocrine Societyen_US
dc.rights© 2013 by The Endocrine Societyen_US
dc.subjectKisspeptinen_US
dc.subjectNeurokinin Ben_US
dc.subjectDynorphin Acten_US
dc.subjectArcuate nucleusen_US
dc.subjectEwesen_US
dc.titleKisspeptin, neurokinin B, and dynorphin cct in the arcuate nucleus to control activity of the GnRH pulse generator in Ewesen_US
dc.typeArticleen_US

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