High-resolution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations

dc.contributor.authorAlaba, Oluwafemi A.
dc.contributor.authorBredeson, Jessen V.
dc.contributor.authorEgesi, Chiedozie N.
dc.contributor.authorEsuma, Williams
dc.contributor.authorEzenwaka, Lydia
dc.contributor.authorFerguson, Morag E.
dc.contributor.authorHa, Cindy M.
dc.contributor.authorHall, Megan
dc.contributor.authorHerselman, Liezel
dc.contributor.authorIkpan, Andrew
dc.contributor.authorKafiriti, Elly
dc.contributor.authorKanju, Edward
dc.contributor.authorKarugu, Arthur
dc.contributor.authorKawuki, Robert
dc.contributor.authorKimata, Bernadetha
dc.contributor.authorKimurto, Paul
dc.contributor.authorKulakow, Peter
dc.contributor.authorKulembeka, Heneriko
dc.contributor.authorKusolwa, Paul
dc.contributor.authorLyons, Jessica B.
dc.contributor.authorMasumba, Esther
dc.contributor.authorVan de Merwe, Albe
dc.contributor.authorMkamilo, Geoffrey
dc.contributor.authorMyburg, Alexander Andrew
dc.contributor.authorNwaogu, Ahamefule
dc.contributor.authorNzuki, Inosters
dc.contributor.authorOlasanmi, Bunmi
dc.contributor.authorOkogbenin, Emmanuel
dc.contributor.authorOwuoche, James O.
dc.contributor.authorPariyo, Anthony
dc.contributor.authorProchnik, Simon E.
dc.contributor.authorRabbi, Ismail Y.
dc.contributor.authorRokhsar, Daniel S.
dc.contributor.authorRounsley, Steve
dc.contributor.authorSalum, Kasele
dc.contributor.authorShuaibu, Kahya S.
dc.contributor.authorSichalwe, Caroline
dc.contributor.authorStephen, Mary
dc.date.accessioned2015-12-11T05:44:32Z
dc.date.available2015-12-11T05:44:32Z
dc.date.issued2015-01
dc.description.abstractCassava (Manihot esculenta Crantz) is a major staple crop in Africa, Asia, and South America, and its starchy roots provide nourishment for 800 million people worldwide. Although native to South America, cassava was brought to Africa 400–500 years ago and is now widely cultivated across sub-Saharan Africa, but it is subject to biotic and abiotic stresses. To assist in the rapid identification of markers for pathogen resistance and crop traits, and to accelerate breeding programs, we generated a framework map for M. esculenta Crantz from reduced representation sequencing [genotyping-by-sequencing (GBS)]. The composite 2412-cM map integrates 10 biparental maps (comprising 3480 meioses) and organizes 22,403 genetic markers on 18 chromosomes, in agreement with the observed karyotype. We used the map to anchor 71.9% of the draft genome assembly and 90.7% of the predicted protein-coding genes. The chromosome-anchored genome sequence will be useful for breeding improvement by assisting in the rapid identification of markers linked to important traits, and in providing a framework for genomic selectionenhanced breeding of this important crop.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipBill and Melinda Gates Foundation (BMGF) Grant OPPGD1493. University of Arizona. CGIAR Research Program on Roots, Tubers, and Bananas. Next Generation Cassava Breeding grant OPP1048542 from BMGF and the United Kingdom Department for International Development. BMGF grant OPPGD1016 to IITA. National Institutes of Health S10 Instrumentation Grants S10RR029668 and S10RR027303.en_ZA
dc.description.urihttp://www.g3journal.orgen_ZA
dc.identifier.citationAlaba, OA, Bredeson, JV, Egesi, CN, Esuma, W, Ezenwaka, L, Ferguson, ME, Ha, CM, Hall, M., Herselman, L, Ikpan, A, Kafiriti, E, Kanju, E, Kapinga, F, Karugu, A, Kawuki, R, Kimata, B, Kimurto, P, Kulakow, P, Kulembeka, H, Kusolwa, P, Lyons, JB, Masumba, E, Van de Merwe, A, Mkamilo, G, Myburg, AA, Nwaogu, A, Nzuki, I, Olasanmi, B, Okogbenin, E, Onyegbule, O, Owuoche, JO, Pariyo, A., Prochnik, SE, Rabbi, IY, Rokhsar, DS, Rounsley, S, Salum, K, Shuaibu, KS, Sichalwe, C, Stephen, M 2015, 'High-resolution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populations', G3 : Genes, Genomes, Genetics, vol. 5, no. 1, pp. 133-144.en_ZA
dc.identifier.issn2160-1836 (online)
dc.identifier.other10.1534/g3.114.015008/-/DC1
dc.identifier.urihttp://hdl.handle.net/2263/51140
dc.language.isoenen_ZA
dc.publisherGenetics Society of Americaen_ZA
dc.rights© 2015 International Cassava Genetic Map Consortium (ICGMC).This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/ by/3.0/).en_ZA
dc.subjectGenotypingby-sequencingen_ZA
dc.subjectCompositeen_ZA
dc.subjectGenetic mapen_ZA
dc.subjectPseudomoleculesen_ZA
dc.subjectF1 crossen_ZA
dc.subjectGenotyping-by-sequencing (GBS)en_ZA
dc.subjectSingle nucleotide polymorphism (SNP)en_ZA
dc.titleHigh-resolution linkage map and chromosome-scale genome assembly for cassava (Manihot esculenta Crantz) from 10 populationsen_ZA
dc.typeArticleen_ZA

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