Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa

dc.contributor.authorMoabelo, Khomotso C.
dc.contributor.authorGcebe, Nomakorinte
dc.contributor.authorGana, James
dc.contributor.authorNgoshe, Yusuf Bitrus
dc.contributor.authorAdesiyun, Abiodun Adewale
dc.contributor.emailabiodun.adesiyun@up.ac.zaen_US
dc.date.accessioned2024-01-15T06:00:56Z
dc.date.available2024-01-15T06:00:56Z
dc.date.issued2023-10
dc.descriptionDATA AVAILABILITY STATEMENT : The data that support the findings of this study are available on request from the corresponding author.en_US
dc.descriptionDATA S1. Supporting Information.en_US
dc.description.abstractThis study determined the prevalence, risk factors, and molecular characteristics of Listeria species detected in beef and beef products sampled in Mpumalanga province, South Africa. Four hundred beef and beef products were collected from 30 retail outlets in three districts (Bronkhorstspruit, Emalahleni, and Middelburg) within the province. Standard bacteriological and polymerase chain reaction (PCR) assays were used in the study. The overall prevalence of L. monocytogenes and other Listeria spp. in the samples was 8.3% (33/400) and 30% (120/400) (p < .05), respectively. For the five variables investigated, statistically significant effects were evident only for the region (p < .001) and type of product (p < .0001) for L. monocytogenes, the type of outlet (p = .011) and the type of product (p < .0001) for Listeria spp. Of the 20 types of beef and beef products tested, 15 (75%) and 17 (85%) were contaminated by L. monocytogenes and Listeria spp., respectively (p = .429). Among the four categories of products tested, the prevalence of L. monocytogenes was 7.3% (8/109), 10.6% (11/104), 7.5% (8/106), and 7.4% (6/81) for raw beef, ready-to-eat (RTE) products, milled beef, and offal & organs, respectively (p > .799). Among the 33 L. monocytogenes isolates, PCR genoserogroup IIa (42.4%, 1/2a-3a) was most frequently detected. All (100%) of the isolates carried one or more of the eight virulence-associated genes assessed, with genes inlC and inlJ detected in all the isolates. The overall prevalence of L. monocytogenes (8.3%) and the high frequency of virulent serogroups of L. monocytogenes commonly associated with human listeriosis pose a food safety risk to consumers of beef and beef-based products contaminated by L. monocytogenes.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.librarianhj2023en_US
dc.description.sdgSDG-02:Zero Hungeren_US
dc.description.sponsorshipRed Meat Research and Development South Africa.en_US
dc.description.urihttp://wileyonlinelibrary.com/journal/jfsen_US
dc.identifier.citationMoabelo, K.C., Gcebe, N., Gana, J., Ngoshe, Y.B., & Adesiyun, A.A. (2023). Contamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africa. Journal of Food Safety, 43(5), e13055. https://doi.org/10.1111/jfs.13055.en_US
dc.identifier.issn0149-6085 (print)
dc.identifier.issn1745-4565 (online)
dc.identifier.other10.1111/jfs.13055
dc.identifier.urihttp://hdl.handle.net/2263/93948
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2023 The Authors. Journal of Food Safety published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License.en_US
dc.subjectPrevalenceen_US
dc.subjectRisk factorsen_US
dc.subjectMolecular characteristicsen_US
dc.subjectListeria speciesen_US
dc.subjectBeef productsen_US
dc.subjectMpumalanga Province, South Africaen_US
dc.subjectContaminationen_US
dc.subjectSDG-02: Zero hungeren_US
dc.titleContamination of beef and beef products by Listeria spp. and molecular characterization of L. monocytogenes in Mpumalanga, South Africaen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Moabelo_Contamination_2023.pdf
Size:
1.57 MB
Format:
Adobe Portable Document Format
Description:
Article
Loading...
Thumbnail Image
Name:
Moabelo_ContaminationSupplData_2023.pdf
Size:
378.24 KB
Format:
Adobe Portable Document Format
Description:
Supplementary Data

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: