Analysis of the spatio-temporal dynamics of a Rho-GEF-H1-myosin activator-inhibitor reaction-diffusion system

dc.contributor.authorMapfumo, Kudzanayi Zebedia
dc.contributor.authorJuma, Victor Ogesa
dc.contributor.authorYigit, Gulsemay
dc.contributor.authorMuchatibaya, Gift
dc.contributor.authorMadzvamuse, Anotida
dc.date.accessioned2025-07-03T11:22:22Z
dc.date.available2025-07-03T11:22:22Z
dc.date.issued2025-04
dc.description.abstractThis study presents a detailed mathematical analysis of the spatio-temporal dynamics of the RhoA-GEF-H1-myosin signalling network, modelled as a coupled system of reaction-diffusion equations. By employing conservation laws and the quasi-steady state approximation, the dynamics is reduced to a tractable nonlinear system. First, we analyse the temporal system of ordinary differential equations (ODE) in the absence of spatial variation, characterizing stability, bifurcations and oscillatory behaviour through phase-plane analysis and bifurcation theory. As parameter values change, the temporal system transitions between stable dynamics; unstable steady states characterized by oscillatory dynamics; and co-existence between locally stable steady states, or co-existence between a locally stable steady state and a locally stable limit cycle. Second, we extend the analysis to the reaction-diffusion system by incorporating diffusion to the temporal ODE model, leading to a comprehensive study of Turing instabilities and spatial pattern formation. In particular, by adding appropriate diffusion to the temporal model: (i) the uniform steady state can be destabilized leading to the well-known Turing diffusion-driven instability (DDI); (ii) one of the uniform stable steady states in the bistable region can be driven unstable, while the other one remains stable, leading to the formation of travelling wave fronts; and (iii) a stable limit cycle can undergo DDI leading to the formation of spatial patterns. More importantly, the interplay between bistability and diffusion shows how travelling wavefronts can emerge, consistent with experimental observations of cellular contractility pulses. Theoretical results are supported by numerical simulations, providing key insights into the parameter spaces that govern pattern transitions and diffusion-driven instabilities.
dc.description.departmentMathematics and Applied Mathematics
dc.description.librarianhj2025
dc.description.sdgNone
dc.description.sponsorshipThe Canada Research Chair (Tier1) in Theoretical and Computational Biology, the Natural Sciences and Engineering Research Council of Canada (NSERC), Discovery Grants Program, the British Columbia Knowledge Development Fund (BCKDF), Canada Foundation for Innovation–JohnR. Evans Leaders Fund–Partnerships (CFI-JELF), the British Columbia Foundation for Non-Animal Research, and the UKRI Engineering and Physical Sciences Research Council.
dc.description.urihttp://royalsocietypublishing.org/journal/rsos
dc.identifier.citationMapfumo, K.Z., Juma, V.O., Yigit, G., Muchatibaya, G. & Madzvamuse, A. Analysis of the spatio-temporal dynamics of a Rho-GEF-H1-myosin activator-inhibitor reaction-diffusion system', Royal Society Open Science, vol. 12, no. 4, art. 241077, doi : 10.1098/rsos.241077.
dc.identifier.issn2054-5703 (online)
dc.identifier.other10.1098/rsos.241077
dc.identifier.urihttp://hdl.handle.net/2263/103160
dc.language.isoen
dc.publisherRoyal Society
dc.rights© 2025 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/.
dc.subjectOrdinary differential equations (ODE)
dc.subjectTravelling wave front
dc.subjectReaction-diffusion
dc.subjectRho-GEF-Myosin signalling network
dc.subjectTuring diffusion-driven instability
dc.subjectActivator-inhibitor system
dc.subjectBifurcation analysis
dc.titleAnalysis of the spatio-temporal dynamics of a Rho-GEF-H1-myosin activator-inhibitor reaction-diffusion system
dc.typeArticle

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