The isolation and identification of an antigen for the diagnosis of bovine mastitis by radial immunodiffusion

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Authors

Viljoen, M.H.

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Publisher

Pretoria : The Government Printer

Abstract

An antigen associated with the somatic cells obtained from mastitic milk, which is also present in the whey and can be used for the diagnosis of mastitis in a radial immunodiffusion (RID) test, has been purified and identified. It is water soluble, with an approximate pI of 4,4 and S ₂₀w =4,35 S. The antigen was shown to be immunologically identical to bovine serum albumin by means of Ouchterlony and immunodiffusion techniques. The use of monospecific rabbit antiserum facilitates standardization of the test results

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Keywords

Veterinary medicine

Sustainable Development Goals

Citation

Viljoen, MH 1974, 'The isolation and identification of an antigen for the diagnosis of bovine mastitis by radial immunodiffusion’, Onderstepoort Journal of Veterinary Research, vol. 41, no. 3, pp. 93-96.