Cytotoxicity, antifungal and antioxidant properties of Lonchocarpus capassa leaf extracts

dc.contributor.authorMachaba, Tambudzani C.
dc.contributor.authorMahlo, Salome M.
dc.contributor.authorEloff, Jacobus Nicolaas
dc.contributor.authorNxumalo, Winston
dc.contributor.authorMcGaw, Lyndy Joy
dc.date.accessioned2024-10-30T11:49:10Z
dc.date.available2024-10-30T11:49:10Z
dc.date.issued2024-02
dc.descriptionSections of this manuscript (12%) are published in a thesis submitted in partial fulfilment of the requirements for the degree of PhD and Masters in the University of Limpopo and University of Pretoria entitled ‘isolation, characterisation and cytotoxicity of antifungal compounds present in medicinal plants used against cryptococcus neoformans in vhembe district, limpopo province’ and ‘Ethnobotanical survey of medicinal plants with antifungal activities in Makhado Local Municipality, Limpopo Province, South Africa’ respectively with Supervisor: Prof. S. Mahlo, available here: http:// ulspace.ul.ac.za/bitstream/handle/10386/4262/makhado_ tc_2018.pdf?isAllowed= y&sequence=1 and http://ulspace. ul.ac.za/bitstream/handle/10386/4291/machaba_tc_2023. pdf?sequence=1&isAllowed=y.en_US
dc.descriptionDATA AVAILABILITY : The data used to support the findings of this study is available from the corresponding author, S.M., upon reasonable request.en_US
dc.description.abstractBACKGROUND : Lonchocarpus capassa is a medicinal plant used to treat diseases such as fungal infections, diarrhoea, oral candidiasis, and stomach complaints in South Africa. AIM : The study aimed to investigate the cytotoxicity and antifungal compounds isolated from L. capassa leaf extracts. SETTING : The study was conducted in Muduluni village, Makhado Local Municipality, Limpopo province. METHODS : Leaf extracts were screened for antifungal activity against fungal pathogens: Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Bioassay-guided fractionation using column chromatography of the acetone extract led to the isolation of six antifungal compounds. Nuclear Magnetic Resonance spectroscopy and Mass Spectrometry were used for the identification of compounds. The antioxidant activity of the plant extracts was investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. Cytotoxicity of isolated compounds was determined using the 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay against Vero monkey kidney cells. RESULTS : The plant extract had an excellent minimum inhibitory concentrations (MIC) value of 40 μg/mL against the microorganisms. Compound 1 was identified as Lupeol, Compound 3 as Friedelin, and Compound 4 as 6-(γ,γ-Dimethylallyl)-3’,4’-dimethoxy-6”,6”-dimethylpyrano-[2”,3”:7,8]-flavanone (Compound 4). Compounds 2 and 5 were not identified because of the presence of mixtures of long-chain fatty acids. Friedelin was the most active radical scavenger in the DPPH assay. The compounds were not toxic with an LC50 value of ˃ 0.2 mg/mL. CONCLUSION : Screening of medicinal plants could provide lead to the discovery of novel antifungal agents. CONTRIBUTION : The results support the traditional use of L. capassa to combat fungal infections in humans.en_US
dc.description.departmentParaclinical Sciencesen_US
dc.description.librarianhj2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe National Research Foundation (NRF).en_US
dc.description.urihttp://www.jomped.org/en_US
dc.identifier.citationMachaba, T.C., Mahlo, S., Eloff, J., Nxumalo, W. & McGaw, L., 2024, ‘Cytotoxicity, antifungal and antioxidant properties of Lonchocarpus capassa leaf extracts’, Journal of Medicinal Plants for Economic Development 8(1), a221. https://doi.org/10.4102/jomped.v8i1.221.en_US
dc.identifier.issn2519-559X (print)
dc.identifier.issn2616-4809 (online)
dc.identifier.other10.4102/jomped.v8i1.221
dc.identifier.urihttp://hdl.handle.net/2263/98847
dc.language.isoenen_US
dc.publisherAOSISen_US
dc.rights© 2024. The Author(s). Licensee: AOSIS. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.en_US
dc.subjectLonchocarpus capassaen_US
dc.subjectAntifungal activityen_US
dc.subjectCytotoxicityen_US
dc.subjectMinimum inhibitory concentration (MIC)en_US
dc.subjectFungal infectionsen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleCytotoxicity, antifungal and antioxidant properties of Lonchocarpus capassa leaf extractsen_US
dc.typeArticleen_US

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