Microbats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortin

dc.contributor.authorChawana, R.
dc.contributor.authorAlagaili, Abdulaziz N.
dc.contributor.authorPatzke, Nina
dc.contributor.authorMohammed, Osama B.
dc.contributor.authorKaswera, Consolate
dc.contributor.authorGilissen, Emmanuel
dc.contributor.authorBennett, Nigel Charles
dc.contributor.authorIhunwo, Amadi O.
dc.contributor.authorManger, Paul R.
dc.contributor.authorSpocter, M.A.
dc.date.accessioned2015-11-18T05:46:25Z
dc.date.available2015-11-18T05:46:25Z
dc.date.issued2014-09
dc.description.abstractA previous study investigating potential adult hippocampal neurogenesis in microchiropteran bats failed to reveal a strong presence of this neural trait. As microchiropterans have a high field metabolic rate and a small body mass, it is possible that capture/handling stress may lead to a decrease in the detectable presence of adult hippocampal neurogenesis. Here we looked for evidence of adult hippocampal neurogenesis using immunohistochemical techniques for the endogenous marker doublecortin (DCX) in 10 species of microchiropterans euthanized and perfusion fixed at specific time points following capture. Our results reveal that when euthanized and perfused within 15 min of capture, abundant putative adult hippocampal neurogenesis could be detected using DCX immunohistochemistry. Between 15 and 30 min post-capture, the detectable levels of DCX dropped dramatically and after 30 min post-capture, immunohistochemistry for DCX could not reveal any significant evidence of putative adult hippocampal neurogenesis. Thus, as with all other mammals studied to date apart from cetaceans, bats, including both microchiropterans and megachiropterans, appear to exhibit substantial levels of adult hippocampal neurogenesis. The present study underscores the concept that, as with laboratory experiments, studies conducted on wild-caught animals need to be cognizant of the fact that acute stress (capture/handling) may induce major changes in the appearance of specific neural traitsen_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipSouth African National Research Foundation (PRM), the Swiss-South African Joint Research Program (AOI and PRM), the Deanship of Scientific Research at the King Saud University through the research group project number RGP_VPP_020 (A.A.), the Belgian co-operation service (DGD) at the Royal Museum for Central Africa (EG), and by a fellowship within the Postdoctoral-Program of the German Academic Exchange Service, DAAD (NP).en_ZA
dc.description.urihttp://www.journals.elsevier.com/neuroscienceen_ZA
dc.identifier.citationChawana, R, Alagaili, AA, Patzke, N, Spocter, MA, Mohammed, OB, Kaswera, C, Gilissen, E, Bennett, NC, Ihunwo, AO & Manger, PR 2014, 'Microbats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortin', Neuroscience, vol. 277, pp. 724-733.en_ZA
dc.identifier.issn0306-4522 (print)
dc.identifier.issn1873-7544 (online)
dc.identifier.other10.1016/j.neuroscience.2014.07.063
dc.identifier.urihttp://hdl.handle.net/2263/50496
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2014 IBRO. Published by Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Neuroscience. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Neuroscience, vol. 277, pp. 724-733, 2014, doi : 10.1016/j.neuroscience.2014.07.063.en_ZA
dc.subjectChiropteraen_ZA
dc.subjectAdult neurogenesisen_ZA
dc.subjectCapture stressen_ZA
dc.subjectDoublecortinen_ZA
dc.subjectFree-living animalsen_ZA
dc.subjectHippocampusen_ZA
dc.subjectBovine serum albumin (BSA)en_ZA
dc.subjectDiaminobenzidine (DAB)en_ZA
dc.subjectDoublecortin (DCX)en_ZA
dc.subjectDoublecortin immunopositive (DCX+)en_ZA
dc.subjectGranular cell layer (GCL)en_ZA
dc.subjectNormal rabbit serum (NRS)en_ZA
dc.subjectPhosphate buffer (PB)en_ZA
dc.subjectSubventricular zone (SVZ)en_ZA
dc.titleMicrobats appear to have adult hippocampal neurogenesis, but post-capture stress causes a rapid decline in the number of neurons expressing doublecortinen_ZA
dc.typePostprint Articleen_ZA

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