Serological differentiation of five bluetongue virus serotypes in indirect ELISA
| dc.contributor.author | Du Plessis, D.H. | |
| dc.contributor.editor | Verwoerd, Daniel Wynand | |
| dc.date.accessioned | 2014-08-21T06:47:01Z | |
| dc.date.available | 2014-08-21T06:47:01Z | |
| dc.date.created | 2013 | |
| dc.date.issued | 1992 | |
| dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
| dc.description.abstract | The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific for virus particles was able to distinguish heterologous serotypes from each other, but a sheep serum from an infected animal was not. An antiserum directed against VP2, the protein responsible for serotype specificity in neutralization tests, was not serotype-specific in ELISA and cross-reacted with other serotypes. The discriminatory ability of a BTV-4 antiserum was improved by cross-absorption with heterologous antigens. This greatly reduced the ELISA signals with heterologous serotypes and produced an antiserum that was effectively serotype-specific. | en |
| dc.description.librarian | mn2014 | |
| dc.identifier.citation | Du Plessis, DH 1992, 'Serological differentiation of five bluetongue virus serotypes in indirect ELISA'. Onderstepoort Journal of Veterinary Research, vol. 59, no. 2, pp. 119-122. | en |
| dc.identifier.issn | 0330-2465 | |
| dc.identifier.uri | http://hdl.handle.net/2263/41461 | |
| dc.language.iso | en | en |
| dc.publisher | Published by the Agricultural Research Council, Onderstepoort Veterinary Institute. | en |
| dc.rights | ©South Africa. Dept. of Agricultural Technical Services. ©University of Pretoria. Dept. of Library Services (digital). | en |
| dc.subject | Veterinary medicine | en |
| dc.subject | BTV | en |
| dc.subject.lcsh | Veterinary medicine -- South Africa | |
| dc.title | Serological differentiation of five bluetongue virus serotypes in indirect ELISA | en |
| dc.type | Article | en |