Increased abundance of secreted hydrolytic enzymes and secondary metabolite gene clusters define the genomes of latent plant pathogens in the Botryosphaeriaceae

dc.contributor.authorNagel, Jan Hendrik
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorSlippers, Bernard
dc.contributor.emailjan.nagel@fabi.up.ac.zaen_ZA
dc.date.accessioned2021-09-02T09:34:18Z
dc.date.available2021-09-02T09:34:18Z
dc.date.issued2021-08
dc.description.abstractBACKGROUND: The Botryosphaeriaceae are important plant pathogens, but also have the ability to establish asymptomatic infections that persist for extended periods in a latent state. In this study, we used comparative genome analyses to shed light on the genetic basis of the interactions of these fungi with their plant hosts. For this purpose, we characterised secreted hydrolytic enzymes, secondary metabolite biosynthetic gene clusters and general trends in genomic architecture using all available Botryosphaeriaceae genomes, and selected Dothideomycetes genomes. RESULTS: The Botryosphaeriaceae genomes were rich in carbohydrate-active enzymes (CAZymes), proteases, lipases and secondary metabolic biosynthetic gene clusters (BGCs) compared to other Dothideomycete genomes. The genomes of Botryosphaeria, Macrophomina, Lasiodiplodia and Neofusicoccum, in particular, had gene expansions of the major constituents of the secretome, notably CAZymes involved in plant cell wall degradation. The Botryosphaeriaceae genomes were shown to have moderate to high GC contents and most had low levels of repetitive DNA. The genomes were not compartmentalized based on gene and repeat densities, but genes of secreted enzymes were slightly more abundant in gene-sparse regions. CONCLUSION: The abundance of secreted hydrolytic enzymes and secondary metabolite BGCs in the genomes of Botryosphaeria, Macrophomina, Lasiodiplodia, and Neofusicoccum were similar to those in necrotrophic plant pathogens and some endophytes of woody plants. The results provide a foundation for comparative genomic analyses and hypotheses to explore the mechanisms underlying Botryosphaeriaceae host-plant interactions.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianpm2021en_ZA
dc.description.sponsorshipThe South African National Research Foundation (NRF), the University of Pretoria and the Department of Science and Innovation (DSI)/National Research Foundation (NRF) Centre of Excellence in Tree Health Biotechnology.en_ZA
dc.description.urihttp://www.biomedcentral.com/bmcgenomicsen_ZA
dc.identifier.citationNagel, J.H., Wingfield, M.J. & Slippers, B. Increased abundance of secreted hydrolytic enzymes and secondary metabolite gene clusters define the genomes of latent plant pathogens in the Botryosphaeriaceae. BMC Genomics 22, 589 (2021). https://doi.org/10.1186/s12864-021-07902-w.en_ZA
dc.identifier.issn1471-2164 (online)
dc.identifier.other10.1186/s12864-021-07902-w
dc.identifier.urihttp://hdl.handle.net/2263/81620
dc.language.isoenen_ZA
dc.publisherBMCen_ZA
dc.rights© The Author(s) 2021 Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License.en_ZA
dc.subjectSecretomeen_ZA
dc.subjectSecondary metabolismen_ZA
dc.subjectComparative genomicsen_ZA
dc.subjectEndophyteen_ZA
dc.subjectPlant cell walldegrading enzymesen_ZA
dc.subjectCarbohydrate-active enzymes (CAZymes)en_ZA
dc.titleIncreased abundance of secreted hydrolytic enzymes and secondary metabolite gene clusters define the genomes of latent plant pathogens in the Botryosphaeriaceaeen_ZA
dc.typeArticleen_ZA

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