Studies on schistosomiasis. 4. Differential staining of live and dead cercariae after immobilization with physostigmin
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Date
Authors
Van Wyk, Jan Aucamp
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Government Printer, Pretoria
Abstract
A modified eosin technique similar to that used for differential staining of live and dead spermatozoa stained dead cercariae of Schistosoma mattheei red while those that were alive remained colourless and transparent.
Prior to exposure to the dye live cercariae were immobilized with physostigmin, which was better for that purpose than five other substances tested.
The lowest concentration of physostigmin to immobilize cercariae quickly and effectively was found to be 3,3 x 10-⁶ M.
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Van Wyk, JA 1973, 'Studies on schistosomiasis. 4. Differential staining of live and dead cercariae after immobilization with physostigmin’, Onderstepoort Journal of Veterinary Research, vol. 40, no. 1, pp. 23-30.