The use of sensitivity discs in the identification of Campylobacter species

Abstract

Filter paper discs were impregnated with a solution containing 20 mg of triphenyltetrazolium chloride per millilitre, and used in the typing of catalase-positive Campylobacter species. Also used were filter paper discs impregnated with cephalothin at 30µg/ml, 60µg/ml and 3 mg/ml and nalidixic acid at the same concentrations, as well as commercially available discs containing 30µg of, respectively, cephalothin and nalidixic acid. Results obtained proved the technique to be reliable and easier to interpret than previously used methods, and laboratory prepared filter paper discs compared favourably with commercial discs.