Biological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteria

dc.contributor.authorChirwa, Evans M.N.
dc.contributor.authorLutsinge Nembudani, Tshilidzi Bridget
dc.contributor.emailevans.chirwa@up.ac.zaen_ZA
dc.date.accessioned2019-05-15T10:04:33Z
dc.date.available2019-05-15T10:04:33Z
dc.date.issued2018
dc.description.abstractThe dissolution and degradation of higher ring-number polycyclic aromatic compounds were tested in batch and attached growth medium using soil biopile and fixed-film bioreactor systems. Bioavailability of polycyclic aromatic hydrocarbons (PAHs) decreases with increasing ring-number. This means high ringnumber compounds will survive longer in the environment and will therefore have a longer-term impact. In the current study, pyrene and fluoranthene were degraded using a biosurfactant producing culture of Pseudomonas aeruginosa grown in fixed-bed and soil biopile experiments. High ring-number PAHs prove to be recalcitrant in nature due to their hydrophobicity and poor partitioning to soil media fixed biomass in biofilm reactors. The use of biosurfactant could facilitate faster dissolution of the PAH and thus would increase bioavailability of the PAH compounds to PAH degrading bacteria. The culture used in this study was obtained from engine oil contaminated soil, that grow on HMW PAHs and are capable of degrading PAHs were enriched, isolated and characterize. The isolated microbial cultures were further characterised 16S rDNA gene sequencing and genotype fingerprinting. During baseline study, over 90 % fluoranthene was degraded as a model compound in biofilm reactor system. Over 88 % pyrene was degraded in soil biopiles. In both cases, increased biosurfactant dose improved degradation rate up to the point where the PAH concentration became inhibitory to biomass growth and PAH degradation.en_ZA
dc.description.departmentChemical Engineeringen_ZA
dc.description.librarianam2019en_ZA
dc.description.urihttp://www.aidic.it/ceten_ZA
dc.identifier.citationChirwa E.M.N., Lutsinge T.B., 2018, Biological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteria , Chemical Engineering Transactions, 70, 1777-1782, DOI: 10.3303/CET1870297.en_ZA
dc.identifier.issn2283-9216 (online)
dc.identifier.other10.3303/CET1870297
dc.identifier.urihttp://hdl.handle.net/2263/69134
dc.language.isoenen_ZA
dc.publisherThe Italian Association of Chemical Engineeringen_ZA
dc.rights© 2018, AIDIC Servizi S.r.l.en_ZA
dc.subjectPseudomonas aeruginosaen_ZA
dc.subjectBiosurfactanten_ZA
dc.subjectBacteriaen_ZA
dc.subjectPolycyclic aromatic hydrocarbon (PAH)en_ZA
dc.titleBiological degradation of pyrene and fluoranthene in porous media and attached growth systems by biosurfactant producing bacteriaen_ZA
dc.typeArticleen_ZA

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