The effect of early rounds of ex vivo expansion and cryopreservation on the adipogenic differentiation capacity of adipose-derived stromal/stem cells
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Date
Authors
Durandt, Chrisna
Dessels, Carla
Da Silva, C.
Murdoch, Candice
Pepper, Michael Sean
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract
Multipotent adipose-derived stromal/stem cells (ASCs) are candidates for use in cellular therapies for
the treatment of a variety of conditions/diseases. Ex vivo expansion of freshly isolated ASCs may be
necessary prior to clinical application to ensure that clinically relevant cell numbers are administered
during treatment. In addition, cryopreserving cells at early passages allows for storage of freshly
isolated cells for extended periods of time before expanding these cells for clinical usage. There are
however several concerns that these laboratory-based procedures may alter the characteristics of the
cells and in so doing decrease their regenerative potential. In this study we report on the impact of
early rounds of cryopreservation (P0) and ex vivo expansion (P0 to P5) on the phenotypic characteristics
and adipogenic differentiation potential of ASCs. Our results show that ASCs that upregulate CD36
expression during adipogenic differentiation gradually decrease with increasing expansion rounds. The
consequent decrease in adipogenic differentiation capacity was evident in both gene expression and
flow cytometry-based phenotypic studies. Successive rounds of expansion did not however alter cell
surface marker expression of the cells. We also show that early cryopreservation of ASCs (at P0) does
not affect the adipogenic differentiation potential of the cells.
Description
Keywords
Ex vivo expansion, Treatment, Cells, Adipose-derived stromal/stem cells (ASCs)
Sustainable Development Goals
Citation
Durandt, C., Dessels, C., Da Silva, C. et al. 2019, 'The effect of early rounds of ex vivo expansion and cryopreservation on the adipogenic differentiation capacity of adipose-derived stromal/stem cells', Scientific Reports, vol. 9, art. 15943, pp. 1-13.