Isolation and potential for transmission of Mycobacterium bovis at human-livestock-wildlife interface of the Serengeti ecosystem, northern Tanzania

dc.contributor.authorKatale, B.Z.
dc.contributor.authorMbugi, Erasto V.
dc.contributor.authorSiame, K.K.
dc.contributor.authorKeyyu, J.D.
dc.contributor.authorKendall, Sharon L.
dc.contributor.authorKazwala, R.R.
dc.contributor.authorDockrell, H.M.
dc.contributor.authorFyumagwa, R.D.
dc.contributor.authorMichel, Anita Luise
dc.contributor.authorRweyemamu, M.
dc.contributor.authorStreicher, E.M.
dc.contributor.authorWarren, R.M.
dc.contributor.authorVan Helden, Paul David
dc.contributor.authorMatee, M.I.
dc.date.accessioned2016-10-31T09:24:47Z
dc.date.available2016-10-31T09:24:47Z
dc.date.issued2017-06
dc.description.abstractMycobacterium bovis, the causative agent of bovine tuberculosis (bTB), is a multihost pathogen of public health and veterinary importance. We characterized the M. bovis isolated at the human–livestock–wildlife interface of the Serengeti ecosystem to determine the epidemiology and risk of cross-species transmission between interacting hosts species. DNA was extracted from mycobacterial cultures obtained from sputum samples of 472 tuberculosis (TB) suspected patients and tissue samples from 606 livestock and wild animal species. M. bovis isolates were characterized using spoligotyping and Mycobacterial Interspersed Repetitive Units-Variable Tandem Repeats (MIRU-VNTR) on 24 loci. Only 5 M. bovis were isolated from the cultured samples. Spoligotyping results revealed that three M. bovis isolates from two buffaloes (Syncerus caffer) and 1 African civet (Civettictis civetta) belonged to SB0133 spoligotype. The two novel strains (AR1 and AR2) assigned as spoligotype SB2290 and SB2289, respectively, were identified from indigenous cattle (Bos indicus). No M. bovis was detected from patients with clinical signs consistent with TB. Of the 606 animal tissue specimens and sputa of 472 TB-suspected patients 43 (7.09%) and 12 (2.9%), respectively, yielded non-tuberculous mycobacteria (NTM), of which 20 isolates were M. intracellulare. No M. avium was identified. M. bovis isolates from wildlife had 45.2% and 96.8% spoligotype pattern agreement with AR1 and AR2 strains, respectively. This finding indicates that bTB infections in wild animals and cattle were epidemiologically related. Of the 24 MIRU-VNTR loci, QUB 11b showed the highest discrimination among the M. bovis strains. The novel strains obtained in this study have not been previously reported in the area, but no clear evidence for recent cross-species transmission of M. bovis was found between human, livestock and wild animals.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipWellcome Trust Grant [WT087546MA] to the Southern African Centre for Infectious Diseases and Surveillance (SACIDS).en_ZA
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1865-1682en_ZA
dc.identifier.citationKatale, BZ, Mbugi, EV, Siame, KK, Keyyu, JD, Kendall, S, Kazwala, RR, Dockrell, HM, Fyumagwa, RD, Michel, AL, Rweyemamu, M, Streicher, EM., Warren, RM, Van Helden, P & Matee, MI 2017, 'Isolation and potential for transmission of Mycobacterium bovis at human-livestock-wildlife interface of the Serengeti ecosystem, northern Tanzania, Transboundary and Emerging Diseases, vol. 64, no. 3, pp. 815-825.en_ZA
dc.identifier.issn1865-1674 (print)
dc.identifier.issn1865-1682 (online)
dc.identifier.other10.1111/tbed.12445
dc.identifier.urihttp://hdl.handle.net/2263/57555
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2015 The Authors. This is an open access article under the terms of the Creative Commons Attribution License.en_ZA
dc.subjectMycobacterium bovisen_ZA
dc.subjectSpoligotypeen_ZA
dc.subjectHuman–animal interfaceen_ZA
dc.subjectSerengeti ecosystemen_ZA
dc.subjectBovine tuberculosis (bTB)en_ZA
dc.subjectTuberculosis (TB)en_ZA
dc.subjectMycobacterial interspersed repetitive units-variable tandem repeats (MIRU-VNTR)en_ZA
dc.titleIsolation and potential for transmission of Mycobacterium bovis at human-livestock-wildlife interface of the Serengeti ecosystem, northern Tanzaniaen_ZA
dc.typePostprint Articleen_ZA

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