Short communication : effects of a 17-beta estradiol analogue on gene expression and morphology in a breast epithelial adenocarcinoma cell line : a potential antiproliferative agent

dc.contributor.authorVisagie, M.H. (Michelle Helen)
dc.contributor.authorStander, Barend Andre
dc.contributor.authorBirkholtz, Lyn-Marie
dc.contributor.authorJoubert, Annie M.
dc.date.accessioned2015-03-09T10:08:26Z
dc.date.available2015-03-09T10:08:26Z
dc.date.issued2013
dc.description.abstract2-Methoxyestradiol is a 17-beta estradiol derivative with antitumor activity. Due to low bioavailability, a new estrogen analogue was in silico-designed. The latter, a sulphamoylated analogue of 2-methoxyestradiol, was evaluated for its potential antiproliferative effect by means of expression microarray analysis and transmission electron microscopy in the highly metastatic breast adenocarcinoma (MDA-MB-231) cell line. Data indicate changes in gene expression pertaining to induction of apoptosis and autophagy as types of cell death, arrest of the cell cycle, and impairment of the cytoskeleton and induction of reactive oxygen species. Transmission electron microscopy confirmed morphological characteristics of apoptosis and autophagy. Altered cell cycle- and cytoskeletal-related gene expression profiles demonstrated that the estradiol analogue acts as an antimitotic agent in this highly metastatic breast cell line. Data also showed that the newly designed estrogen analogue exerts an antiproliferative effect in this cancer cell line culminating in both apoptosis and autophagy as type of cell death paving the way for further investigations into its potential as anticancer agent.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipCancer Association of South Africa, the Struwig Germeshuysen Trust, RESCOM (Research Council of the University of Pretoria), National Research Foundation and The Medical Research Council.en_ZA
dc.description.urihttp://www.biomedres.infoen_ZA
dc.identifier.citationVisagie MH, Stander BA, Birkholtz L-M, Joubert AM 2013, 'Short communication : effects of a 17-beta estradiol analogue on gene expression and morphology in a breast epithelial adenocarcinoma cell line : a potential antiproliferative agent', Biomedical Research, vol. 24, no. 4, pp. 525-530.en_ZA
dc.identifier.issn0388-6107 (print)
dc.identifier.issn1880-313X (online)
dc.identifier.urihttp://hdl.handle.net/2263/43890
dc.language.isoenen_ZA
dc.publisherBiomedical Research Pressen_ZA
dc.rights© 2013 Biomedical Research Pressen_ZA
dc.subjectApoptosisen_ZA
dc.subjectMicroarrayen_ZA
dc.subjectAutophagyen_ZA
dc.subjectAntimitoticen_ZA
dc.subjectCell cycleen_ZA
dc.titleShort communication : effects of a 17-beta estradiol analogue on gene expression and morphology in a breast epithelial adenocarcinoma cell line : a potential antiproliferative agenten_ZA
dc.typeArticleen_ZA

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