Effect of calcium soil amendments on phenolic compounds and soft rot resistance in potato tubers
| dc.contributor.author | Ngadze, Elizabeth | |
| dc.contributor.author | Coutinho, Teresa A. | |
| dc.contributor.author | Icishahayo, David | |
| dc.contributor.author | Van der Waals, Jacqueline Elise | |
| dc.contributor.email | jacquie.vdwaals@up.ac.za | en_US |
| dc.date.accessioned | 2014-07-15T10:05:11Z | |
| dc.date.available | 2014-07-15T10:05:11Z | |
| dc.date.issued | 2014-08 | |
| dc.description.abstract | Nutrients such as boron, nitrogen and calcium stimulate the production of phenols. This research focuses on the role of calcium in increasing phenol metabolism in potato peels and the ensuing tuber resistance to soft rot pathogens was investigated. Two field experiments were conducted at the University of Zimbabwe campus plots in 2008 and 2009 summer seasons. Sprouted tubers of cv. BP1 were planted in plots treated with different fertilizer combinations. The treatments were: 1) compound S (7N: 21P: 8K) þ ammonium nitrate (34 %N) 2) compound D (7N: 14P: 7K) þ calcium nitrate (19 Ca: 15.5N) 3) compound S þ calcium nitrate and 4) compound D þ ammonium nitrate. The harvested progeny tubers were inoculated with Pectobacterium carotovorum subsp. brasiliense. Calcium amendment increased the activities of enzymes (phenylalanine ammonia-lyase, polyphenol oxidase and peroxidases) involved in the metabolism of phenolics and total soluble phenols. Calcium amendment significantly reduced (P < 0.05) the maceration effect of P. carotovorum subsp. brasiliense in tuber tissues. Chlorogenic acid, caffeic acid and calcium, but not ferulic acid content were significantly higher (P < 0.05) in plants grown in calcium treated plots. Calcium positively and significantly correlated with polyphenol oxidase (PPO), phenylalanine (PAL) and peroxidase (POD), while chlorogenic and caffeic acid showed a positive relationship with POD and PPO. Calcium amendment significantly reduced maceration symptom caused by the bacteria (P < 0.05), resulting in smaller decayed zone diameters on inoculated tubers from calciumtreated plots. This study shows that soil amendments of calcium increase concentration of calcium, caffeic and chlorogenic acid in tuber peels and also reduces maceration effect of pectinolytic pathogens. Reduced maceration could be due to increased levels of caffeic and chlorogenic acid which have antimicrobial properties. | en_US |
| dc.description.librarian | hb2014 | en_US |
| dc.description.uri | http://www.elsevier.com/locate/cropro | en_US |
| dc.identifier.citation | Ngadze, E, Coutinho, TA, Icishahayo, D & Van der Waals, JE 2014, 'Effect of calcium soil amendments on phenolic compounds and soft rot resistance in potato tubers', Crop Protection, vol. 62, pp. 40-45. | en_US |
| dc.identifier.issn | 0261-2194 (print) | |
| dc.identifier.issn | 1873-6904 (online) | |
| dc.identifier.other | 10.1016/j.cropro.2014.04.009 | |
| dc.identifier.uri | http://hdl.handle.net/2263/40764 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.rights | © 2014 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Crop Protection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Crop Protection, vol. 62, pp. 40-45, 2014. doi : 10.1016/j.cropro.2014.04.009. | en_US |
| dc.subject | Peroxidases | en_US |
| dc.subject | Polyphenol oxidase | en_US |
| dc.subject | Phenylalanine ammonia lyase | en_US |
| dc.subject | Solanum tuberosum | en_US |
| dc.title | Effect of calcium soil amendments on phenolic compounds and soft rot resistance in potato tubers | en_US |
| dc.type | Postprint Article | en_US |