Novel species of Huntiella from naturally-occurring forest trees in Greece and South Africa
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Date
Authors
Liu, FeiFei
Marincowitz, Seonju
Chen, ShuaiFei
Mbenoun, Michael
Tsopelas, Panaghiotis
Soulioti, Nikoleta
Wingfield, Michael J.
Journal Title
Journal ISSN
Volume Title
Publisher
Pensoft Publishers
Abstract
Huntiella species are wood-infecting, filamentous ascomycetes that occur in fresh wounds on a wide variety
of tree species. These fungi are mainly known as saprobes although some have been associated with
disease symptoms. Six fungal isolates with typical culture characteristics of Huntiella spp. were collected
from wounds on native forest trees in Greece and South Africa. The aim of this study was to identify these
isolates, using morphological characters and multigene phylogenies of the rRNA internal transcribed
spacer (ITS) region, portions of the β-tubulin (BT1) and translation elongation factor 1α (TEF-1α) genes.
The mating strategies of these fungi were also determined through PCR amplification of mating type
genes. The study revealed two new species; one from Platanus orientalis in Greece and one from Colophospermum
mopane and Senegalia nigrescens in South Africa. These novel taxa have been provided with the
names, H. hellenica sp. nov. and H. krugeri sp. nov., respectively. The former species was found to have a
homothallic and the latter a heterothallic mating system.
Description
Supplementary material 1 : Figure S1. ML tree of Huntiella species generated from the ITS DNA sequence data
Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun,
Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield
Data type: phylogenetic tree
Explanation note: Sequences generated from this study are printed in bold type. Bold
branches indicate posterior probabilities values ≥ 0.9. Bootstrap values and posterior probabilities value are presented above branches as ML/MP/BI. Bootstrap
value < 50% or probabilities values < 0.9 are marked with *. Nodes lacking the
support value are marked with -. Ceratocystis cercfabiensis (CMW 43029) represents
the outgroup.
Copyright notice: This dataset is made available under the Open Database License
(http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License
(ODbL) is a license agreement intended to allow users to freely share, modify, and
use this Dataset while maintaining this same freedom for others, provided that the
original source and author(s) are credited.
Link: https://doi.org/10.3897/mycokeys.69.53205.suppl1
Supplementary material 2 : Figure S2. ML tree of Huntiella species generated from the BT1 DNA sequence data Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun, Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield Data type: phylogenetic tree Explanation note: Sequences generated from this study are printed in bold type. Bold branches indicate posterior probabilities values ≥ 0.9. Bootstrap values and posterior probabilities values are presented above branches as ML/MP/BI. Bootstrap value < 50% or probabilities values < 0.9 are marked with *. Nodes lacking the support value are marked with -. Ceratocystis cercfabiensis (CMW 43029) represents the outgroup. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.69.53205.suppl2 52 FeiFei Liu et al. / MycoKeys 69: 33–52 (2020)
Supplementary material 3 : Figure S3. ML tree of Huntiella species generated from the TEF-1α DNA sequence data Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun, Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield Data type: phylogenetic tree Explanation note: Sequences generated from this study are printed in bold type. Bold branches indicate posterior probabilities values ≥ 0.9. Bootstrap values and posterior probabilities values are presented above branches as ML/MP/BI. Bootstrap value < 50% or probabilities values < 0.9 are marked with *. Nodes lacking the support value are marked with -. Ceratocystis cercfabiensis (CMW 43029) represents the outgroup. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.69.53205.suppl3
Supplementary material 2 : Figure S2. ML tree of Huntiella species generated from the BT1 DNA sequence data Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun, Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield Data type: phylogenetic tree Explanation note: Sequences generated from this study are printed in bold type. Bold branches indicate posterior probabilities values ≥ 0.9. Bootstrap values and posterior probabilities values are presented above branches as ML/MP/BI. Bootstrap value < 50% or probabilities values < 0.9 are marked with *. Nodes lacking the support value are marked with -. Ceratocystis cercfabiensis (CMW 43029) represents the outgroup. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.69.53205.suppl2 52 FeiFei Liu et al. / MycoKeys 69: 33–52 (2020)
Supplementary material 3 : Figure S3. ML tree of Huntiella species generated from the TEF-1α DNA sequence data Authors: FeiFei Liu, Seonju Marincowitz, ShuaiFei Chen, Michael Mbenoun, Panaghiotis Tsopelas, Nikoleta Soulioti, Michael J. Wingfield Data type: phylogenetic tree Explanation note: Sequences generated from this study are printed in bold type. Bold branches indicate posterior probabilities values ≥ 0.9. Bootstrap values and posterior probabilities values are presented above branches as ML/MP/BI. Bootstrap value < 50% or probabilities values < 0.9 are marked with *. Nodes lacking the support value are marked with -. Ceratocystis cercfabiensis (CMW 43029) represents the outgroup. Copyright notice: This dataset is made available under the Open Database License (http://opendatacommons.org/licenses/odbl/1.0/). The Open Database License (ODbL) is a license agreement intended to allow users to freely share, modify, and use this Dataset while maintaining this same freedom for others, provided that the original source and author(s) are credited. Link: https://doi.org/10.3897/mycokeys.69.53205.suppl3
Keywords
Ceratocystidaceae, Ceratocystis moniliformis complex, Colophospermum mopane, Huntiella, Platanus orientalis, Saprobes, Senegalia nigrescens, β-tubulin (BT1), Translation elongation factor 1α (TEF-1α), Internal transcribed spacer (ITS), Polymerase chain reaction (PCR)
Sustainable Development Goals
Citation
Liu FF, Marincowitz S, Chen SF, Mbenoun M, Tsopelas P, Soulioti N, Wingfield MJ (2020) Novel species
of Huntiella from naturally-occurring forest trees in Greece and South Africa. MycoKeys 69: 33–52. https://DOI.org/ 10.3897/mycokeys.69.53205.