The effect of three complexes of iodine with amino acids on gene expression of model antibiotic resistant microorganisms Escherichia coli ATCC BAA-196 and Staphylococcus aureus ATCC BAA-39

dc.contributor.authorKenesheva, Sabina T.
dc.contributor.authorTaukobong, Setshaba
dc.contributor.authorShilov, Sergey V.
dc.contributor.authorKuznetsova, Tatyana V.
dc.contributor.authorJumagaziyeva, Ardak B.
dc.contributor.authorKarpenyuk, Tatyana A.
dc.contributor.authorReva, Oleg N.
dc.contributor.authorIlin, Aleksandr I.
dc.contributor.emailoleg.reva@up.ac.zaen_US
dc.date.accessioned2024-08-27T06:54:21Z
dc.date.available2024-08-27T06:54:21Z
dc.date.issued2023-07
dc.descriptionDATA AVAILABILITY STATEMENT : The RNA reads generated for this study are available from the NCBI SRA database through the BioProjects PRJNA557356 and PRJNA480363 created, respectively, for the genomes of E. coli ATCC BAA-196 and S. aureus ATCC BAA-39. The links to the respective SRA Experiments are available on the BioProjectWeb-pages. The predicted X-ray structures of the complexes KS25, KS33, and KS51 were deposited at the CCDC database (https://www.ccdc.cam.ac. uk/, accessed on 26 June 2023) under accession numbers 1036607, 1036667, and 1436137, respectively.en_US
dc.descriptionSUPPLEMENTARY INFORMATION : SUPPLEMENTARY TABLE S1. X-ray diffraction and refinement parameters; SUPPLEMENTARY TABLE S2. Genes regulated with statistical reliability (|fold change| 2.0; p-value 0.05) under the effect of iodine-containing complexes at least at one condition; SUPPLEMENTARY TABLE S3. Regulation of expression of genes involved in the central metabolism of bacteria by the three tested complexes.en_US
dc.description.abstractBACKGROUND : Iodine is a broad-spectrum antimicrobial disinfectant for topical application. Recent studies have shown promising results on the applicability of an iodine-containing complex, FS-1, against antibiotic-resistant pathogens. It was hypothesized that the antimicrobial activity of iodine-containing complexes may be modulated by the organic moiety of the complex, i.e., amino acids. METHODS : Gene regulation and metabolic alterations were studied in two model multidrug-resistant microorganisms, Staphylococcus aureus ATCC BAA-39, and Escherichia coli ATCC BAA- 196, treated with three complexes containing iodine and three different amino acids: glycine, Lalanine, and L-isoleucine. The bacterial cultures were exposed to sub-lethal concentrations of the complexes in the lagging and logarithmic growth phases. Gene regulation was studied by total RNA sequencing and differential gene expression analysis. RESULTS : The central metabolism of the treated bacteria was affected. An analysis of the regulation of genes involved in stress responses suggested the disruption of cell wall integrity, DNA damage, and oxidative stress in the treated bacteria. CONCLUSIONS : Previous studies showed that the application of iodine-containing complexes, such as FS-1, serves as a supplement to common antibiotics and can be a promising way to combat antibiotic-resistant pathogens. Current results shed light on possible mechanisms of this action by disrupting the cell wall barriers and imposing oxidative stress. It was also found that the effect of the complexes on metabolic pathways varied in the tested microorganisms depending on the organic moiety of the complexes and the growth phase when the complexes had been applied.en_US
dc.description.departmentBiochemistryen_US
dc.description.departmentGeneticsen_US
dc.description.departmentMicrobiology and Plant Pathologyen_US
dc.description.librarianam2024en_US
dc.description.sdgSDG-03:Good heatlh and well-beingen_US
dc.description.sponsorshipThe Ministry of Industry and Infrastructural Development of the Republic of Kazakhstan and the National Research Foundation (NRF) of South Africa.en_US
dc.description.urihttps://www.mdpi.com/journal/microorganismsen_US
dc.identifier.citationKenesheva, S.T.; Taukobong, S.; Shilov, S.V.; Kuznetsova, T.V.; Jumagaziyeva, A.B.; Karpenyuk, T.A.; Reva, O.N.; Ilin, A.I. The Effect of Three Complexes of Iodine with Amino Acids on Gene Expression of Model Antibiotic Resistant Microorganisms Escherichia coli ATCC BAA-196 and Staphylococcus aureus ATCC BAA-39. Microorganisms 2023, 11, 1705. https://DOI.org/10.3390/microorganisms11071705.en_US
dc.identifier.issn2076-2607 (online)
dc.identifier.other10.3390/microorganisms11071705
dc.identifier.urihttp://hdl.handle.net/2263/97883
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rights© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.en_US
dc.subjectRNA sequencingen_US
dc.subjectTranscriptomicsen_US
dc.subjectAntibiotic resistanceen_US
dc.subjectEscherichia colien_US
dc.subjectStaphylococcus aureusen_US
dc.subjectIodineen_US
dc.subjectAmino acidsen_US
dc.subjectSDG-03: Good health and well-beingen_US
dc.titleThe effect of three complexes of iodine with amino acids on gene expression of model antibiotic resistant microorganisms Escherichia coli ATCC BAA-196 and Staphylococcus aureus ATCC BAA-39en_US
dc.typeArticleen_US

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