A multicomponent, elicitor-inducible cystatin complex in tomato, Solanum lycopersicum

dc.contributor.authorGirard, Cécile
dc.contributor.authorRivard, Daniel
dc.contributor.authorKiggundu, Andrew
dc.contributor.authorKunert, Karl J.
dc.contributor.authorGleddie, Stephen C.
dc.contributor.authorCloutier, Conrad
dc.contributor.authorMichaud, Dominique
dc.date.accessioned2007-08-30T06:26:59Z
dc.date.available2007-08-30T06:26:59Z
dc.date.issued2007-03
dc.description.abstract*We assessed the ability of the fungal elicitor arachidonic acid to induce cystatin genes in tomato (Solanum lycopersicum), using a cDNA expression library from arachidonate-treated leaves. *The cDNAs of two novel cystatins were isolated, coding for an approx. 11-kDa protein, SlCYS10; and for a 23.6-kDa protein, SlCYS9, bearing an N-terminal signal peptide and a long, 11.5-kDa extension at the C terminus. Both genes were induced by arachidonate but not by methyl jasmonate, an inducer of the 88-kDa eight-unit cystatin, multicystatin, accumulated in the cytosol of leaf cells upon herbivory. *A truncated form of SlCYS9, tSlCYS9, was produced by deletion of the C-terminal extension to assess the influence of this structural element on the cystatin moiety. As shown by kinetic and stability assays with recombinant variants expressed in Escherichia coli, deleting the extension influenced both the overall stability and inhibitory potency of SlCYS9 against cysteine proteases of herbivorous organisms. *These findings provide evidence for a multicomponent elicitor-inducible cystatin complex in tomato, including at least 10 cystatin units produced via two metabolic routes.en
dc.description.sponsorshipThis work was supported by the Natural Science and Engineering Research Council of Canada, and by the Fonds Québécois pour la Recherche sur la Nature et les Technologies (Québec). C.G. was the recipient of a postdoctoral fellowship from the Centre de Recherche en Horticulture of Laval University. A.K. was the recipient of a fellowship from the Rockefeller Foundation.en
dc.format.extent531437 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationGirard, C, Rivard, D, Kiggundu, A, Kunert, K, Gleddie, SC, Cloutier, C & Michaud, D 2007, ‘A multicomponent, elicitor-inducible cystatin complex in tomato, Solanum lycopersicum’, New Phytologist, vol. 173, issue 4, pp. 841-851 [http://www.blackwell-synergy.com]en
dc.identifier.issn1469-8137
dc.identifier.other10.1111/j.1469-8137.2007.01968.x
dc.identifier.urihttp://hdl.handle.net/2263/3381
dc.language.isoenen
dc.publisherBlackwellen
dc.rightsBlackwell. The definitive version of the article is available at www.balckwell-synergy.com.en
dc.subjectSolanum lycopersicumen
dc.subject.lcshTomatoes -- Genetics
dc.subject.lcshArachidonic acid
dc.subject.lcshPlant genetics
dc.titleA multicomponent, elicitor-inducible cystatin complex in tomato, Solanum lycopersicumen
dc.typePostprint Articleen

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