Development of the OPgun™ for bombardment of animal tissues
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Date
Authors
Brayton, Kelly A.
Bothma, G.C.
Vogel, S.W.
Allsopp, B.A.
Journal Title
Journal ISSN
Volume Title
Publisher
Published by the Agricultural Research Council, Onderstepoort Veterinary Institute
Abstract
A simple and inexpensive particle-bombardment device, the OPgun™, was constructed for the
delivery of DNA into animal tissues. This device is based on the particle-inflow gun first described
for plant-cell transfection. The delivery of tungsten particles into the epidermis of the mouse ear,
without the use of vacuum and without causing damage to the tissue, was demonstrated. The system
was also shown to be capable of inducing antibodies to a foreign gene in mice.
Description
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Keywords
Veterinary medicine
Sustainable Development Goals
Citation
Brayton, KA, Bothma, GC, Vogel, SW & Allsopp, BA 1997, 'Development of the OPgun™ for bombardment of animal tissues’, Onderstepoort Journal of Veterinary Research, vol. 64, no. 2, pp. 153-156.