In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells
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Date
Authors
Nkandeu, Danielle Sandra
Mqoco, Thandi Vuyelwa
Visagie, Michelle Helen
Stander, Barend Andre
Wolmarans, Elize
Cronje, Marianne J.
Joubert, Annie M.
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley
Abstract
2-Methoxyestradiol, a natural metabolite of estradiol, exerts antiproliferative and antitumour properties in vitro and in vivo. Because of its low
oral bioavailability, several promising analogues of 2-methoxyestradiol have been developed. In this study, the in vitro influence of the
compound, 2-ethyl-3-O-sulphamoyl-estra-1,3,5(10)16-tetraene (C19), a non-commercially available 17-b-estradiol analogue, was tested on
the breast adenocarcinoma MCF-7 cell line. The in vitro influence of 24 h exposure to 0.18 mM of C19 on MCF-7 cells was evaluated on
cell morphology, cell cycle progression and possible induction of apoptosis and autophagy. Polarization-optical transmitted light differential
interference contrast and fluorescence microscopy revealed the presence of cells blocked in metaphase, occurrence of apoptotic bodies and
compromised cell density in C19-treated cells. Hallmarks of autophagy, namely an increase in the number of acidic vacuoles and lysosomes,
were also observed in C19-treated samples. An increase in the number of cells present in the sub-G1 fraction, as well as a reduction in
mitochondrial membrane potential was observed. No significant alterations in caspase 8 activity were observed. A twofold increase in
aggresome formation was observed in C19-treated cells. C19 induced both apoptosis and autophagy in MCF-7 cells.
Description
Keywords
2-Methoxyestradiol (2ME2), MCF-7, Autophagy, Apoptosis
Sustainable Development Goals
Citation
Nkandeu, DS, Mqoco, TV, Visagie, MH, Stander, BA, Wolmarans, E, Cronje, MJ & Joubert, AM 2013, 'In vitro changes in mitochondrial potential, aggresome formation and caspase activity by a novel 17-beta-estradiol analogue in breast adenocarcinoma cells', Cell Biochemistry and Function, vol. 31, no. 7, pp. 566-574.