Antibiotic-potentiation, antioxidant, cytotoxic, anti-inflammatory and anti-acetylcholinesterase potential of Antidesma madagascariense Lam. (Euphorbiaceae)

dc.contributor.authorSeebaluck-Sandoram, Roumita
dc.contributor.authorLall, Namrita
dc.contributor.authorFibrich, Bianca
dc.contributor.authorBlom van Staden, Analike
dc.contributor.authorMahomoodally, Fawzi
dc.date.accessioned2017-04-24T10:35:03Z
dc.date.issued2017-07
dc.description.abstractAntidesma madagascariense Lam. (AM) is used in the treatment and management of a panoply of human diseases. Leaf decoction, acetone extracts and fractions of AM were evaluated for antimicrobial, antioxidant, anti-inflammatory, cytotoxicity and acetylcholinesterase (AChE) inhibitory activity. The antibiotic potentiating activity of crude acetone extract (AE) was assessed against ATCC bacterial strains of Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923 using standard antibiotics. Decoction extract showed significant inhibitory activity against Acinetobacter spp. (MIC: 0.25 mg/mL) which had higher inhibitory effect compared to the positive control Streptomycin (MIC: 1.00 mg/mL). Acetone and decoction extracts inhibited the growth of Candida albicans (MIC: 4.00 mg/mL). All fractions of AE showed broad spectrum activity against ATCC and clinical strains (MIC 0.03–4.00 mg/mL). The results of the combination pro- files of the AE with Ciprofloxacin, Chloramphenicol and Streptomycin revealed synergistic interactions (FICI ≤ 0.50) against P. aeruginosa. Crude decoction, acetone extract and fractions were found to be potent reducing agent as well DPPH radical scavenger and ABTS cation scavenger. A significant correlation between DPPH, ABTS and total phenolic content (p b 0.05, r=−0.75 and −0.82 respectively) was recorded. Three fractions namely DCM:MeOH (85:15) (F5), DCM:MeOH (95:05) (F3) and DCM (F2) [IC50 0.02–0.09 mg/mL] demonstrated significant anti-inflammatory activity as compared to the positive control, diclofenac sodium [IC50 0.18 (0.10–0.31) mg/mL]. AE and its fractions showed AChE inhibitory activities at an IC50 of 35.92–492.6 μg/mL. Cytotoxicity study against Vero cells revealed that AE and hexane fraction were non-cytotoxic while decoction showed cytotoxic effect. Further studies are required to explore the potential of AM crude extracts and fractions as natural source of antimicrobial, antioxidant, anti-inflammatory agents as well as AChE inhibitors.en_ZA
dc.description.departmentPlant Production and Soil Scienceen_ZA
dc.description.embargo2018-07-31
dc.description.librarianhb2017en_ZA
dc.description.urihttp://www.elsevier.com/locate/sajben_ZA
dc.identifier.citationSeebaluck-Sandoram, R, Lall, N, Fibrich, B, Blom van Staden, A & Mahomoodally, F 2017, 'Antibiotic-potentiation, antioxidant, cytotoxic, anti-inflammatory and anti-acetylcholinesterase potential of Antidesma madagascariense Lam. (Euphorbiaceae)', South African Journal of Botany, vol. 111, pp. 194-201.en_ZA
dc.identifier.issn0254-6299 (print)
dc.identifier.issn1727-9321 (online)
dc.identifier.other10.1016/j.sajb.2017.03.034
dc.identifier.urihttp://hdl.handle.net/2263/60023
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.rights© 2017 SAAB. Published by Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in South African Journal of Botany. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in South African Journal of Botany, vol. 111, pp. 194-201, 2017. doi : 10.1016/j.sajb.2017.03.034.en_ZA
dc.subjectAntimicrobialen_ZA
dc.subjectAntibiotic-potentiatingen_ZA
dc.subjectAntioxidanten_ZA
dc.subjectAnti-inflammatoryen_ZA
dc.subjectCytotoxicityen_ZA
dc.subjectAcetylcholinesteraseen_ZA
dc.subjectMauritiusen_ZA
dc.subjectAntidesma madagascariense (AM)en_ZA
dc.titleAntibiotic-potentiation, antioxidant, cytotoxic, anti-inflammatory and anti-acetylcholinesterase potential of Antidesma madagascariense Lam. (Euphorbiaceae)en_ZA
dc.typePostprint Articleen_ZA

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
SeebaluckSandoram_AntibioticPotentiation_2017.pdf
Size:
498.05 KB
Format:
Adobe Portable Document Format
Description:
Post-print Article

License bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
license.txt
Size:
1.75 KB
Format:
Item-specific license agreed upon to submission
Description: