Putative novel Bradyrhizobium and Phyllobacterium species isolatedfrom root nodules of Chamaecytisus ruthenicus

dc.contributor.authorKalita, Michał
dc.contributor.authorMałek, Wanda
dc.contributor.authorCoutinho, Teresa A.
dc.date.accessioned2020-06-03T12:56:19Z
dc.date.available2020-06-03T12:56:19Z
dc.date.issued2020-03
dc.descriptionAppendix A. Supplementary dataen_ZA
dc.description.abstractIn this study, the diversity and the phylogenetic relationships of bacteria isolated from root nodules ofChamaecytisus ruthenicus growing in Poland were investigated using ERIC-PCR fingerprinting and by mul-tilocus sequence analysis (MLSA). Two major clusters comprising 13 and 3 isolates were detected which16S rRNA gene sequencing identified as Bradyrhizobium and Phyllobacterium. The results of phylogeneticanalysis of individual and concatenated atpD, gyrB and recA gene sequences showed that the studiedstrains may represent novel species in the genera Bradyrhizobium and Phyllobacterium. In the phyloge-netic tree based on the atpD-gyrB-recA concatemers, Bradyrhizobium isolates were split into two groupsclosely related to Bradyrhizobium algeriense STM89Tand Bradyrhizobium valentinum LmjM3T. The genusPhyllobacterium isolates formed a separate cluster close to Phyllobacterium ifriqiyense LMG27887Tin theatpD-gyrB-recA phylogram. Analysis of symbiotic gene sequences (nodC, nodZ, nifD, and nifH) showed thatthe Bradyrhizobium isolates were most closely related to Bradyrhizobium algeriense STM89T, Bradyrhizo-bium valentinum LmjM3Tand Bradyrhizobium retamae Ro19Tbelonging to symbiovar retamae. This isthe first report on the occurrence of members of symbiovar retamae from outside the Mediterraneanregion. No symbiosis related genes were amplified from Phyllobacterium strains, which were also unableto induce nodules on C. ruthenicus roots. Based on these findings Phyllobacterium isolates can be regardedas endophytic bacteria inhabitating root nodules of C. ruthenicus.en_ZA
dc.description.departmentBiochemistryen_ZA
dc.description.departmentForestry and Agricultural Biotechnology Institute (FABI)en_ZA
dc.description.departmentGeneticsen_ZA
dc.description.departmentMicrobiology and Plant Pathologyen_ZA
dc.description.librarianam2020en_ZA
dc.description.sponsorshipFaculty of Biotechnology and Biology, Maria Curie-Sklodowska University, Lublin, Poland.en_ZA
dc.description.urihttp://www.elsevier.com/locate/syapmen_ZA
dc.identifier.citationKalita, M., Małek, W. & Coutinho, T.A. 2020, 'Putative novel Bradyrhizobium and Phyllobacterium species isolatedfrom root nodules of Chamaecytisus ruthenicus', Systematic and Applied Microbiolog 43, art. 126056, pp. 1-8.en_ZA
dc.identifier.issn0723-2020
dc.identifier.other10.1016/j.syapm.2020.126056
dc.identifier.urihttp://hdl.handle.net/2263/74854
dc.language.isoenen_ZA
dc.publisherElsevier GmbHen_ZA
dc.rights© 2020 The Author(s). Published by Elsevier GmbH. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en_ZA
dc.subjectBradyrhizobiumen_ZA
dc.subjectPhyllobacteriumen_ZA
dc.subjectNodulation genesen_ZA
dc.subjectHousekeeping genesen_ZA
dc.subjectChamaecytisus ruthenicusaen_ZA
dc.subjectMultilocus sequence analysis (MLSA)en_ZA
dc.subjectERIC-PCR fingerprintingen_ZA
dc.titlePutative novel Bradyrhizobium and Phyllobacterium species isolatedfrom root nodules of Chamaecytisus ruthenicusen_ZA
dc.typeArticleen_ZA

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