Wheat line “RYNO3936” is associated with delayed water stress-induced leaf senescence and rapid water-deficit stress recovery

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Authors

Le Roux, Marlon-Schylor L.
Burger, N. Francois V.
Vlok, Mare
Kunert, Karl J.
Cullis, Christopher A.
Botha, Anna-Maria

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Frontiers Media

Abstract

Random mutagenesis was applied to produce a new wheat mutant (RYNO3926) with superior characteristics regarding tolerance to water deficit stress induced at late booting stage. The mutant also displays rapid recovery from water stress conditions. Under water stress conditions mutant plants reached maturity faster and produced more seeds than its wild type wheat progenitor. Wild-type Tugela DN plants died within 7 days after induction of water stress induced at late booting stage, while mutant plants survived by maintaining a higher relative moisture content (RMC), increased total chlorophyll, and a higher photosynthesis rate and stomatal conductance. Analysis of the proteome of mutant plants revealed that they better regulate post-translational modification (SUMOylation) and have increased expression of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) proteins. Mutant plants also expressed unique proteins associated with dehydration tolerance including abscisic stress-ripening protein, cold induced protein, cold-responsive protein, dehydrin, Group 3 late embryogenesis, and a lipoprotein (LAlv9) belonging to the family of lipocalins. Overall, our results suggest that our new mutant RYNO3936 has a potential for inclusion in future breeding programs to improve drought tolerance under dryland conditions.

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TABLE S2 : Blast2GO results from the peptides obtained after LC-ESI-MS/MS analysis of total protein isolated from RYNO3936 before (day 0), and after induction of water stress (days 7 and 14), and after recovery and regrowth (day 21).
TABLE S3 : List of proteins expressed in RYNO3936 before (day 0), and after induction of water stress (days 7 and 14), as well as after recovery and regrowth (day 21). Indicated are the sequence name, protein identity, length and number of hits, number of GO terms, and e-value. The cluster numbers correspond to that given in Figure S2.
FIGURE S1 : Proportional contribution of proteins expressed in RYNO3936 to the different functional categories, where (A) biological processes; (B) cellular component; and (C) molecular function.
FIGURE S2 : Cluster image generated by Java TreeView (Saldanha, 2004) of the proteins obtained after LC-ESI-MS/MS analysis of total protein isolated from RYNO3936 before (day 0), and after induction of water stress (days 7 and 14), and after recovery and regrowth (day 21). Red bands show up-regulated proteins, whereas green bands show down-regulated proteins.

Keywords

Random mutagenesis, Mutant wheat, Drought, Photosynthesis, Chlorophyll fluorescence, RuBisCO, Relative moisture content (RMC), Liquid chromatography–mass spectrometry (LC-MS)

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Citation

Le Roux, M.-S.L., Burger, N.F.V., Vlok, M., Kunert, K.J., Cullis, C.A. and Botha, A.-M. (2020) Wheat Line “RYNO3936” Is Associated With Delayed Water Stress-Induced Leaf Senescence and Rapid Water-Deficit Stress Recovery. Frontiers in Plant Science 11:1053. DOI: 10.3389/fpls.2020.01053.