B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera

dc.contributor.authorMathebula, Evans Mantiri
dc.contributor.authorFaber, Frederika E.
dc.contributor.authorVan Wyngaardt, Wouter
dc.contributor.authorVan Schalkwyk, Antoinette
dc.contributor.authorPretorius, Alri
dc.contributor.authorFehrsen, Jeanni
dc.date.accessioned2017-04-03T13:19:31Z
dc.date.available2017-04-03T13:19:31Z
dc.date.issued2017-02-24
dc.description.abstractIdentifying antigenic proteins and mapping their epitopes is important for the development of diagnostic reagents and recombinant vaccines. B-cell epitopes of African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive study of the humoral immune response of five vaccinated horses to AHSV-4 antigenic peptides was undertaken. A fragmented-genome phage display library expressing a repertoire of AHSV-4 peptides spanning the entire genome was constructed. The library was affinity selected for binders on immobilised polyclonal immunoglobulin G (IgG) isolated from horse sera collected pre- and post-immunisation with an attenuated AHSV-4 monovalent vaccine. The DNA inserts of binding phages were sequenced with Illumina high-throughput sequencing. The data were normalised using preimmune IgG-selected sequences. More sequences mapped to the genes coding for NS3, VP6 and VP5 than to the other genes. However, VP2 and VP5 each had more antigenic regions than each of the other proteins. This study identified a number of epitopes to which the horse’s humoral immune system responds during immunisation with AHSV-4.en_ZA
dc.description.departmentVeterinary Tropical Diseasesen_ZA
dc.description.librarianam2017en_ZA
dc.description.sponsorshipThe Economic Competitiveness Support Programme, ARC-OVI, South Africaen_ZA
dc.description.urihttp://www.ojvr.orgen_ZA
dc.identifier.citationMathebula, E.M., Faber, F.E., Van Wyngaardt, W., Van Schalkwyk, A., Pretorius, A. & Fehrsen, J., 2017, ‘B-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse sera’, Onderstepoort Journal of Veterinary Research 84(1), a1313. https://DOI.org/ 10.4102/ojvr.v84i1.1313.en_ZA
dc.identifier.issn0030-2465 (print)
dc.identifier.issn2219-0635 (online)
dc.identifier.other10.4102/ojvr.v84i1.1313
dc.identifier.urihttp://hdl.handle.net/2263/59641
dc.language.isoenen_ZA
dc.publisherAOSIS OpenJournalsen_ZA
dc.rights© 2017. The Authors. Licensee: AOSIS. This work is licensed under the Creative Commons Attribution License.en_ZA
dc.subjectProteinsen_ZA
dc.subjectEpitopesen_ZA
dc.subjectVaccineen_ZA
dc.subjectAfrican horse sickness virus (AHSV)en_ZA
dc.titleB-cell epitopes of African horse sickness virus serotype 4 recognised by immune horse seraen_ZA
dc.typeArticleen_ZA

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