Generation and characterization of monoclonal antibodies against Rift Valley fever virus nucleoprotein

dc.contributor.authorFafetine, Jose Manuel
dc.contributor.authorDomingos, Ana
dc.contributor.authorAntunes, Sandra
dc.contributor.authorEsteves, Aida
dc.contributor.authorPaweska, Janusz Tadeusz
dc.contributor.authorCoetzer, Jacobus A.W.
dc.contributor.authorRutten, Victor P.M.G.
dc.contributor.authorDas Neves, Luis Carlos Bernardo G.
dc.date.accessioned2014-02-20T07:53:04Z
dc.date.available2014-12-01T00:20:05Z
dc.date.issued2013-11
dc.description.abstractDue to the unpredictable and explosive nature of Rift Valley fever (RVF) outbreaks, rapid and accurate diagnostic assays for low-resource settings are urgently needed. To improve existing diagnostic assays, monoclonal antibodies (MAbs) specific for the nucleocapsid protein of RVF virus (RVFV) were produced and characterized. Four IgG2a MAbs showed specific binding to denatured nucleocapsid protein, both from a recombinant source and from inactivated RVFV, in Western blot analysis and in an enzyme-linked immunosorbent assay (ELISA). Cross-reactivity with genetically related and non-related arboviruses including Bunyamwera and Calovo viruses (Bunyaviridae family), West Nile and Dengue-2 viruses (Flaviviridae family), and Sindbis and Chikungunya viruses (Togaviridae family) was not detected. These MAbs represent a useful tool for the development of rapid diagnostic assays for early recognition of RVF.en
dc.description.librarianhb2014en
dc.description.librarianab2014en
dc.description.sponsorshipSIDA/SAREC –Eduardo Mondlane University; the Utrecht University, The Netherlands and Southern African Centre for Infectious Disease Surveillance (SACIDS).en
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1865-1682en
dc.identifier.citationFafetine, JM, Domingos, A, Antunes, S, Esteves, A, Paweska, JT, Coetzer, JAW, Rutten, VPMG & Neves, L 2013, 'Generation and characterization of monoclonal antibodies against Rift Valley fever virus nucleoprotein', Transboundary and Emerging Diseases, vol. 60, Suppl 2, pp. 24-30.en
dc.identifier.issn1865-1674 (print)
dc.identifier.issn1865-1682 (online)
dc.identifier.other10.1111/tbed.12140
dc.identifier.urihttp://hdl.handle.net/2263/33542
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2013 Blackwell Verlag GmbH • Transboundary and Emerging Diseases. The definite version is available at : http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1865-1682en
dc.subjectNucleocapsid proteinen
dc.subjectCross-reactivityen
dc.subject.lcshRift Valley fever (RVF)en
dc.subject.lcshZoonosesen
dc.subject.lcshMonoclonal antibody (MAb)en
dc.titleGeneration and characterization of monoclonal antibodies against Rift Valley fever virus nucleoproteinen
dc.typePostprint Articleen

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