Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells
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Date
Authors
Sahle, M.
Burgess, W.G.
Kidanemariam, Awoke
Journal Title
Journal ISSN
Volume Title
Publisher
Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.
Abstract
This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.
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Keywords
Veterinary medicine, Cell culture, MDBK cell, Newcastle disease virus (NDV), V4 strain replication
Sustainable Development Goals
Citation
Sahle, M, Burgess, WG & Kidanemariam, A 2002, 'Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells’. Onderstepoort Journal of Veterinary Research, vol. 69, no. 3, pp. 201-206