A Malaysia 97 monovalent foot-and-mouth disease vaccine (> 6PD(50)/dose) protects pigs against challenge with a variant FMDV A SEA-97 lineage virus, 4 and 7 days post vaccination

dc.contributor.authorSinganallur, Balasubramanian Nagendrakumar
dc.contributor.authorNguyen, Thi Thu Hong
dc.contributor.authorFosgate, Geoffrey Theodore
dc.contributor.authorMorris, Michelle Jacqueline
dc.contributor.authorDavis, Andrew J.
dc.contributor.authorGiles, Michelle
dc.contributor.authorKim, Van Phuc
dc.contributor.authorQuach, Vo Ngon
dc.contributor.authorLe, Thi Thu Phuong
dc.contributor.authorNguyen, Ngoc Hong Phuc
dc.contributor.authorTran, Xuan Hanh
dc.contributor.authorVo, Van Hung
dc.contributor.authorLe, Thi Quynhanh
dc.contributor.authorTran, Minh Tan
dc.contributor.authorNgo, Thanh Long
dc.contributor.authorVosloo, Wilna
dc.date.accessioned2016-02-29T08:32:21Z
dc.date.available2016-02-29T08:32:21Z
dc.date.issued2015-08
dc.description.abstractPigs play a significant role during outbreaks of foot-and-mouth disease (FMD) due to their ability to amplify the virus. It is therefore essential to determine what role vaccination could play to prevent clinical disease and lower virus excretion into the environment. In this study we investigated the efficacy of the double oil emulsion A Malaysia 97 vaccine (>6PD50/dose) against heterologous challenge with an isolate belonging to the A SEA-97 lineage at 4 and 7 days post vaccination (dpv). In addition, we determined whether physical separation of pigs in the same room could prevent virus transmission. Statistically there was no difference in the level of protection offered by 4 and 7 dpv. However, no clinical disease or viral RNA was detected in the blood of pigs challenged 4 dpv, although three of the pigs had antibodies to the non-structural proteins (NSPs), indicating viral replication. Viral RNA was also detected in nasal and saliva swabs, but on very few occasions. Two of the pigs vaccinated seven days prior to challenge had vesicles distal from the injection site, but on the inoculated foot, and two pigs had viral RNA detected in the blood. One pig sero-converted to the NSPs. In contrast, all unvaccinated and inoculated pigs had evidenceof infection. No infection occurred in any of the susceptible pigs in the same room, but separated from the infected pigs, indicating that strict biosecurity measures were sufficient under these experimental conditions to prevent virus transmission. However, viral RNA was detected in the nasal swabs of one group of pigs, but apparently not at sufficient levels to cause clinical disease. Vaccination led to a significant decrease in viral RNA in vaccinated pigs compared to unvaccinated and infected pigs, even with this heterologous challenge, and could therefore be considered as a control option during outbreaks.en_ZA
dc.description.librarianhb2015en_ZA
dc.description.sponsorshipCattle Council of Australia, Australian Dairy Farmers, Australian Lot Feeders Association, Wool Producers Australia,Sheepmeat Council of Australia, Australian Pork Limited and the Goat Industry Council of Australia through the Meat and Livestock Australia Donor Company matching arrangements with the Australian Government (Project PSH 0558).en_ZA
dc.description.urihttp://www.elsevier.com/locate/vaccineen_ZA
dc.identifier.citationSinganallur, BN, Nguyen, TTH, Fosgate, TG, Morris, MJ, Davis, A, Giles, M, Kim,VP, Quach, VN, Le, TTP, Nguyen, NHP, Tran, XH, Vo, VH, Le, TQ, Tran, MT, Ngo, TL & Vosloo, W 2015, 'A Malaysia 97 monovalent foot-and-mouth disease vaccine (> 6PD(50)/dose) protects pigs against challenge with a variant FMDV A SEA-97 lineage virus, 4 and 7 days post vaccination', Vaccine, vol. 33, pp. 4513-4519.en_ZA
dc.identifier.issn0264-410X (print)
dc.identifier.issn1873-2518 (online)
dc.identifier.other10.1016/j.vaccine.2015.07.014
dc.identifier.urihttp://hdl.handle.net/2263/51583
dc.language.isoenen_ZA
dc.publisherElsevieren_ZA
dc.relation.requiresAdobe Acrobat Readeren
dc.rights© 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_ZA
dc.subjectSerotype Aen_ZA
dc.subjectPigsen_ZA
dc.subjectVaccinationen_ZA
dc.subjectEarly protectionen_ZA
dc.subjectHeterologous challengeen_ZA
dc.subjectFoot-and-mouth diseaseen_ZA
dc.subjectFMDen_ZA
dc.titleA Malaysia 97 monovalent foot-and-mouth disease vaccine (> 6PD(50)/dose) protects pigs against challenge with a variant FMDV A SEA-97 lineage virus, 4 and 7 days post vaccinationen_ZA
dc.typeArticleen_ZA

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