Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate
dc.contributor.author | Taole, M. | |
dc.contributor.author | Albertyn, J. | |
dc.contributor.author | Van Heerden, E. | |
dc.contributor.author | Bragg, R.R. (Robert Richard) | |
dc.contributor.editor | Boomker, Jacob Diederik Frederik | |
dc.date.accessioned | 2012-02-28T07:42:59Z | |
dc.date.available | 2012-02-28T07:42:59Z | |
dc.date.created | 2012 | |
dc.date.issued | 2002 | |
dc.description | The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. | en |
dc.description.abstract | A NAD-dependent isolate 46 (C-3) of Haemophilus paragallinarum, which was previously demonstrated to be of high virulence, was transformed to NAD independence using a plasmid isolated from a naturally occurring NAD-independent isolate of H. paragallinarum. The transformation was performed by two different methods and the identity of all of the isolates, before and after transformation was confirmed using a H. paragallinarum-specific PCR test. The transformed NAD-independent serovar C-3 isolate and the wild-type serovar C-3 isolate were used to experimentally infect vaccinated layer chickens. It was shown that the transformation to NAD independence significantly altered the virulence of the serovar C-3 isolate that was used in the transformation experiment. The mechanisms responsible for a decrease in virulence are not clear, but may be related to the pathology of the transformed isolate in the sinus of the chickens. | en |
dc.description.librarian | mn2012 | en |
dc.identifier.citation | Taole, M, Albertyn, J, Van Heerden, E & Bragg, RR 2002, 'Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate’. Onderstepoort Journal of Veterinary Research, vol. 69, no. 3, pp. 189-196. | en |
dc.identifier.issn | 0030-2465 | |
dc.identifier.uri | http://hdl.handle.net/2263/18254 | |
dc.language | en | |
dc.publisher | Published jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria. | en |
dc.rights | © ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital). | en |
dc.subject | Veterinary medicine | en |
dc.subject | Haemophilus paragallinarum | en |
dc.subject | Infectious coryza | en |
dc.subject | NAD dependent | en |
dc.subject | NAD independent | en |
dc.subject | Transformation | en |
dc.subject.lcsh | Veterinary medicine -- South Africa | |
dc.subject.lcsh | Haemophilus -- South Africa | en |
dc.subject.lcsh | Haemophilus parainfluenzae | en |
dc.title | Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate | en |
dc.type | Article | en |