Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate

dc.contributor.authorTaole, M.
dc.contributor.authorAlbertyn, J.
dc.contributor.authorVan Heerden, E.
dc.contributor.authorBragg, R.R. (Robert Richard)
dc.contributor.editorBoomker, Jacob Diederik Frederik
dc.date.accessioned2012-02-28T07:42:59Z
dc.date.available2012-02-28T07:42:59Z
dc.date.created2012
dc.date.issued2002
dc.descriptionThe articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat v.9 was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.en
dc.description.abstractA NAD-dependent isolate 46 (C-3) of Haemophilus paragallinarum, which was previously demonstrated to be of high virulence, was transformed to NAD independence using a plasmid isolated from a naturally occurring NAD-independent isolate of H. paragallinarum. The transformation was performed by two different methods and the identity of all of the isolates, before and after transformation was confirmed using a H. paragallinarum-specific PCR test. The transformed NAD-independent serovar C-3 isolate and the wild-type serovar C-3 isolate were used to experimentally infect vaccinated layer chickens. It was shown that the transformation to NAD independence significantly altered the virulence of the serovar C-3 isolate that was used in the transformation experiment. The mechanisms responsible for a decrease in virulence are not clear, but may be related to the pathology of the transformed isolate in the sinus of the chickens.en
dc.description.librarianmn2012en
dc.identifier.citationTaole, M, Albertyn, J, Van Heerden, E & Bragg, RR 2002, 'Virulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolate’. Onderstepoort Journal of Veterinary Research, vol. 69, no. 3, pp. 189-196.en
dc.identifier.issn0030-2465
dc.identifier.urihttp://hdl.handle.net/2263/18254
dc.languageen
dc.publisherPublished jointly by the Agricultural Research Council, ARC-Onderstepoort Veterinary Institute and the Faculty of Veterinary Science, University of Pretoria.en
dc.rights© ARC-Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © University of Pretoria. Dept of Library Services (digital).en
dc.subjectVeterinary medicineen
dc.subjectHaemophilus paragallinarumen
dc.subjectInfectious coryzaen
dc.subjectNAD dependenten
dc.subjectNAD independenten
dc.subjectTransformationen
dc.subject.lcshVeterinary medicine -- South Africa
dc.subject.lcshHaemophilus -- South Africaen
dc.subject.lcshHaemophilus parainfluenzaeen
dc.titleVirulence of South African isolates of Haemophilus paragallinarum. Part 3: Experimentally produced NAD-independent isolateen
dc.typeArticleen

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