Large-scale international validation of an indirect ELISA based on recombinant nucleocapsid protein of rift valley fever virus for the detection of IgG antibody in domestic ruminants
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Date
Authors
Paweska, Janusz Tadeusz
Jansen van Vuren, Petrus
Msimang, Veerle
Lo, Modu Moustapha
Thiongane, Yaya
Mulumba-Mfumu, Leopold K.
Mansoor, Alqadasi
Fafetine, Jose M.
Magona, Joseph W.
Boussini, Hiver
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA)
based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated
for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera.
Validation data sets were dichotomized according to the results of a virus neutralization test in sera
obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal,
Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were
defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic
specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6%
(cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7%
(sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international
validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard
incubation and inactivation procedures evaluated did not have an adverse effect on the detectable
levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based
I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried
out outside expensive bio-containment facilities. These advantages are particularly important for
less-resourced countries where there is a need to accelerate and improve RVF surveillance and
research on epidemiology as well as to advance disease control measures.
Description
Keywords
Recombinant nucleocapsid, IgG antibody, Domestic ruminants, Validation, Diagnostic accuracy, Rift Valley fever virus (RVFV), Indirect enzyme-linked immunosorbent assay (I-ELISA)
Sustainable Development Goals
Citation
Pawęska, J.T., van Vuren, P.J., Msimang, V., Lô, M.M., Thiongane, Y., Mulumba-Mfumu, L.K., Mansoor, A., Fafetine, J.M., Magona, J.W., Boussini, H.; et al.
Large-Scale International Validation
of an Indirect ELISA Based on
Recombinant Nucleocapsid Protein of
Rift Valley Fever Virus for the
Detection of IgG Antibody in
Domestic Ruminants. Viruses 2021, 13,
1651. https://doi.org/10.3390/v13081651.