Polymerase chain reaction-based national surveillance programme to determine the distribution and prevalence of Taylorella equigenitalis in South African horses

dc.contributor.authorMay, Catherine Edith
dc.contributor.authorGuthrie, Alan John
dc.contributor.authorKeys, Bronwyn
dc.contributor.authorJoone, Christopher
dc.contributor.authorMonyai, Mpho S.
dc.contributor.authorSchulman, M.L. (Martin)
dc.contributor.emailmartin.schulman@up.ac.zaen_ZA
dc.date.accessioned2016-08-01T08:08:46Z
dc.date.issued2016-05
dc.description.abstractREASONS FOR PERFORMING STUDY : The response to the first outbreak of contagious equine metritis in South Africa included pioneering a web-based platform to coordinate key aspects of a national, real-time polymerase chain reaction (qPCR)-based stallion screening programme to determine the distribution and prevalence of Taylorella equigenitalis in stallions and exposed mares. OBJECTIVES : To define the hypothesised pre-existing status of T. equigenitalis in the South African equine population and progression of the epidemiological investigation via the implementation of a molecular diagnostic-based surveillance programme. STUDY DESIGN : Retrospective case series. METHODS : Screening for T. equigenitalis was via a qPCR assay on genital swabs obtained from predilection sites in stallions and mares with subsequent confirmation using bacterial culture according to prescribed methods.en_ZA
dc.description.departmentCompanion Animal Clinical Studiesen_ZA
dc.description.departmentEquine Research Centreen_ZA
dc.description.departmentProduction Animal Studiesen_ZA
dc.description.embargo2017-05-31
dc.description.librarianhb2016en_ZA
dc.description.sponsorshipEquine Research Centre, University of Pretoria, South Africa and the Department of Agriculture, Forestry and Fisheries, Republic of South Africa.en_ZA
dc.description.urihttp://onlinelibrary.wiley.com/journal/10.1001/(ISSN)2042-3306en_ZA
dc.identifier.citationMay, CE, Guthrie, AJ, Keys, B, Joone, C, Monyai, M & Schulman, ML 2016, 'Polymerase chain reaction-based national surveillance programme to determine the distribution and prevalence of Taylorella equigenitalis in South African horses', Equine Veterinary Journal, vol. 48, no. 3, pp. 307-311.en_ZA
dc.identifier.issn0425-1644
dc.identifier.other10.1111/evj.12439
dc.identifier.other7006535470
dc.identifier.otherJ-6375-2013
dc.identifier.other0000-0001-7729-9918
dc.identifier.urihttp://hdl.handle.net/2263/56152
dc.language.isoenen_ZA
dc.publisherWileyen_ZA
dc.rights© 2015 EVJ Ltd. This is the pre-peer reviewed version of the following article : Polymerase chain reaction-based national surveillance programme to determine the distribution and prevalence of Taylorella equigenitalis in South African horses, Equine Veterinary Journal, vol. 48, no. 3, pp. 307-311, 2016. doi : 10.1111/evj.12439. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1001/(ISSN)2042-3306.en_ZA
dc.subjectHorsesen_ZA
dc.subjectFomite transmissionen_ZA
dc.subjectqPCRen_ZA
dc.subjectTaylorella equigenitalisen_ZA
dc.titlePolymerase chain reaction-based national surveillance programme to determine the distribution and prevalence of Taylorella equigenitalis in South African horsesen_ZA
dc.typePostprint Articleen_ZA

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