Prevalence and characterization of Campylobacter species from chickens sold at informal chicken markets in Gauteng, South Africa

dc.contributor.authorPhosa, Mathie
dc.contributor.authorFasina, Folorunso Oludayo
dc.contributor.authorMorar-Leather, Darshana
dc.contributor.authorAdesiyun, Abiodun Adewale
dc.contributor.emailabiodun.adesiyun@up.ac.zaen_US
dc.date.accessioned2023-05-19T12:41:19Z
dc.date.available2023-05-19T12:41:19Z
dc.date.issued2022-10
dc.description.abstractThis study determined the prevalence, characteristics, and risk factors of Campylobacter species contamination of chicken carcasses sold at informal poultry outlets in Gauteng province, South Africa. Within six townships, 151 chicken carcasses were collected from 47 outlets. Carcass swab, cloacal swab, and carcass drip samples were collected from each chicken, along with a matched questionnaire on risk factors regarding Campylobacter contamination. Sample-inoculated Bolton broth (BB) was cultured to isolate Campylobacter species by bacteriological methods. Subsequent confirmation and characterization of Campylobacter were conducted using polymerase chain reaction (PCR). Isolated Campylobacter strains were evaluated for the presence of six virulence genes (ciaB, dnaj, pldA, racR, flaA, and flaB), three toxin genes (cdtA, cdtB, and cdtC), and one antimicrobial resistance gene (tetO). The overall prevalence of Campylobacter was 23.4% (106 of 453), with sample type–specific prevalence being 17.2% (26 of 151), 25.8% (39 of 151), and 27.2% (41 of 151) for the carcass swabs, cloacal swabs, and carcass drip, respectively, following bacteriological isolation and confirmation by PCR. The overall prevalence of Campylobacter species was 93.5% by PCR, which varied significantly (P = 0.000) by sample: 99.2, 98.4, and 82.8% for carcass swabs, cloacal swabs, and carcass drip, respectively, by using PCR to detect Campylobacter in BB. Important risk factors for carcass contamination by Campylobacter included the slaughter of culled breeders and spent chickens, the use of stagnant water, and poor sanitation. Virulence and toxin gene frequencies were higher in C. jejuni–positive (82.5%) than in C. coli–positive (71.4%) BB cultures, but tetracycline resistance gene (tetO) frequency was higher in C. coli (75.9%) than in C. jejuni (48.10%). The observed high frequencies in C. jejuni recovered from street-vended chickens may pose food safety and therapeutic concerns to consumers.en_US
dc.description.departmentProduction Animal Studiesen_US
dc.description.departmentVeterinary Tropical Diseasesen_US
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe Department of Agriculture and Research Council, the Agriculture Sector Education Training Authority, and the National Research Foundation.en_US
dc.description.urihttps://www.sciencedirect.com/journal/journal-of-food-protectionen_US
dc.identifier.citationPhosa, M., Fasina, F.O., Morar-leather, D. & Adesiyun, A.A. 2022, 'Prevalence and characterization of Campylobacter species from chickens sold at informal chicken markets in Gauteng, South Africa', Journal of Food Protection, vol. 85, no. 10, pp. 1458-1468, doi : 10.4315/JFP-21-454.en_US
dc.identifier.issn0362-028X (print)
dc.identifier.issn1944-9097 (online)
dc.identifier.other10.4315/JFP-21-454
dc.identifier.urihttp://hdl.handle.net/2263/90757
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rights© International Association for Food Protection. Article is published in a open archive.en_US
dc.subjectCampylobacter spp.en_US
dc.subjectChickensen_US
dc.subjectInformal marketen_US
dc.subjectSouth Africa (SA)en_US
dc.subjectToxigenicityen_US
dc.subjectVirulenceen_US
dc.titlePrevalence and characterization of Campylobacter species from chickens sold at informal chicken markets in Gauteng, South Africaen_US
dc.typeArticleen_US

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