Antifungal compounds from the leaves of Rhynchosia minima

dc.contributor.authorAdewole, Adetola Henry
dc.contributor.authorFamuyide, Ibukun Michael
dc.contributor.authorMcGaw, Lyndy Joy
dc.contributor.authorSelepe, Mamoalosi A.
dc.contributor.authorOctober, Natasha
dc.contributor.emailmamoalosi.selepe@up.ac.zaen_US
dc.date.accessioned2023-03-09T08:00:27Z
dc.date.available2023-03-09T08:00:27Z
dc.date.issued2022-12
dc.description.abstractRhynchosia minima, commonly known as jumby bean, is used as a remedy for respiratory ailments in various parts of the world. It is also used by South African traditional healers to treat heart or chest pain. This study aimed to investigate the bioactive constituents of the leaf extracts of R. minima against selected fungal isolates that have been identified as risk factors in respiratory illness. Rhynchosia minima leaves were extracted sequentially using hexane, dichloromethane, ethyl acetate and methanol in increasing order of polarity. The extracts were subjected to repeated chromatographic techniques, for phytochemical isolation. The extracts and isolated compounds were screened against Candida albicans and Cryptococcus neoformans by determining the minimum concentration that inhibited fungal growth. Six flavonoids, one norisoprenoid and one cyclitol were isolated and characterized by 1D and 2D NMR and HR-ESI-MS. The extracts obtained in the study had moderate to weak antifungal activities, with MICs ranging from 312.5 to 1250.0 μg/mL against both fungi. Four isolated compounds were also screened, with two of them exhibiting activity against C. albicans (MIC=6.25 μg/mL) that was comparable to amphotericin B, the positive control. These two compounds also had better antifungal potential against C. neoformans with an MIC=6.25 μg/mL, compared to the MIC of 12.5 μg/mL of amphotericin B. Seven of the eight isolated compounds were obtained from the extracts of Rhynchosia minima for the first time. Two of the isolated compounds demonstrated activity comparable or superior to amphotericin B activity. The notable potency displayed by these compounds warrants further investigation on their development as antifungal agents.en_US
dc.description.departmentChemistryen_US
dc.description.departmentParaclinical Sciencesen_US
dc.description.librarianhj2023en_US
dc.description.sponsorshipThe National Research Foundation, Indigenous Knowledge System) South Africa and the University of Pretoria.en_US
dc.description.urihttps://onlinelibrary.wiley.com/journal/16121880en_US
dc.identifier.citationAdewole, A.H., Famuyide, I.M., McGaw, L.J. et al. 2022, 'Antifungal compounds from the leaves of Rhynchosia minima', Chemistry and Biodiversity, vol. 19, no. 12, art. e202200837, pp. 1-9, doi : 10.1002/cbdv.202200837.en_US
dc.identifier.issn1612-1872 (print)
dc.identifier.issn1612-1880 (online)
dc.identifier.other10.1002/cbdv.202200837
dc.identifier.urihttps://repository.up.ac.za/handle/2263/90042
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rights© 2022 The Authors. Chemistry & Biodiversity published by Wiley-VHCA AG, Zurich, Switzerland. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License.en_US
dc.subjectAntifungal activityen_US
dc.subjectCyclitolen_US
dc.subjectFlavonoidsen_US
dc.subjectNorisoprenoiden_US
dc.subjectJumby bean (Rhynchosia minima)en_US
dc.titleAntifungal compounds from the leaves of Rhynchosia minimaen_US
dc.typeArticleen_US

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