Characterization of Botryosphaeriaceae from plantation-grown Eucalyptus species in South China

dc.contributor.authorChen, ShuaiFei
dc.contributor.authorPavlic-Zupanc, Draginja
dc.contributor.authorRoux, Jolanda
dc.contributor.authorSlippers, Bernard
dc.contributor.authorXie, YaoJian
dc.contributor.authorWingfield, Michael J.
dc.contributor.authorZhou, Xudong
dc.contributor.emailxu.zhou@fabi.up.ac.zaen_US
dc.date.accessioned2011-05-04T09:52:44Z
dc.date.available2011-05-04T09:52:44Z
dc.date.issued2011
dc.description.abstractThe Botryosphaeriaceae is a species-rich family that includes pathogens of a wide variety of trees, including Eucalyptus species. Symptoms typical of infection by the Botryosphaeriaceae have recently been observed in Eucalyptus plantations in South China. The aim of this study was to identify the Botryosphaeriaceae associated with these symptoms. Isolates were collected from branch cankers and senescent twigs of different Eucalyptus spp. All isolates resembling Botryosphaeriaceae were separated into groups based on conidial morphology. Initial identifications were made using PCR-RFLP fingerprinting, by digesting the ITS region of the rDNA operon with the restriction enzymes CfoI and KspI. Furthermore, to distinguish isolates in the Neofusicoccum parvum/N. ribis complex, a locus (BotF15) previously shown to define these species, was amplified and restricted with CfoI. Selected isolates were then identified using comparisons of DNA sequence data for the ITS rDNA and translation elongation factor 1-alpha (TEF-1α) gene regions. Based on anamorph morphology and DNA sequence comparisons, five species were identified: Lasiodiplodia pseudotheobromae, L. theobromae, Neofusicoccum parvum, N. ribis sensu lato and one undescribed taxon, for which the name Fusicoccum fabicercianum sp. nov. is provided. Isolates of all species gave rise to lesions on the stems of an E. grandis clone in a glasshouse inoculation trial and on the stems of five Eucalyptus genotypes inoculated in the field, where L. pseudotheobromae and L. theobromae were most pathogenic. The five Eucalyptus genotypes differed in their susceptibility to the Botryosphaeriaceae species suggesting that breeding and selection offers opportunity for disease avoidance in the future.en
dc.description.sponsorshipBilateral agreement between South Africa and China funded through projects 30771732, 2007DFA31190 and 2006BAD08A11; members of the Tree Protection Co-operative Programme (TPCP)en_US
dc.identifier.citationChen, SF, Pavlic, D, Roux, J, Slippers, B, Xie, YJ, Wingfield, MJ & Zhou, XD 2011, 'Characterization of Botryosphaeriaceae from plantation-grown Eucalyptus species in South China', Plant Pathology, in press. [http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1365-3059]en_US
dc.identifier.issn0032-0862
dc.identifier.issn1365-3059 (online)
dc.identifier.other10.1111/j.1365-3059.2011.02431.x
dc.identifier.urihttp://hdl.handle.net/2263/16452
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.rights© 2011 University of Pretoria © 2011 BSPP. This article is embargoed by the publisher until March 2012.en_US
dc.subjectFusicoccum fabicercianum sp. nov.en
dc.subjectLasiodiplodiaen
dc.subjectNeofusicoccumen
dc.subject.lcshEucalyptus -- Diseases and pests -- Chinaen
dc.subject.lcshBotryosphaeriaceae -- Chinaen
dc.subject.lcshCanker (Plant disease) -- Chinaen
dc.subject.lcshEndophytic fungi -- Chinaen
dc.titleCharacterization of Botryosphaeriaceae from plantation-grown Eucalyptus species in South Chinaen
dc.typePostprint Articleen

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